Fiechter A, Mian F A, Ris H, Halvorson H O
J Bacteriol. 1972 Feb;109(2):855-61. doi: 10.1128/jb.109.2.855-861.1972.
Saccharomyces cerevisiae was grown in a chemostat in the presence of excess oxygen. Cells harvested from fully derepressed and strongly repressed steady states show typical promitochondria-like structures under conditions of strong repression. Insoluble membrane proteins were extracted from highly purified mitochondria and submitted to isoelectric focusing in 6% polyacrylamide gels. Some 20 protein bands were obtained from derepressed cells. The pattern was clearly different (quantitatively and possibly qualitatively) from repressed mitochondria. In contrast to ribosomal proteins, insoluble membrane protein fractions were found in the acid section (pH 4 to 6.8) of the ampholyte gels. It can be concluded that glucose repression plays a prominent role in the synthesis of the functional mitochondrial membranes.
酿酒酵母在恒化器中于氧气过量的条件下培养。从完全去阻遏和强烈阻遏的稳定状态收获的细胞在强烈阻遏条件下显示出典型的类原线粒体结构。从不溶性膜蛋白从高度纯化的线粒体中提取，并在6%聚丙烯酰胺凝胶中进行等电聚焦。从去阻遏细胞中获得了约20条蛋白带。该模式与阻遏线粒体明显不同（在数量上可能在质量上也不同）。与核糖体蛋白相反，在两性电解质凝胶的酸性部分（pH 4至6.8）发现了不溶性膜蛋白组分。可以得出结论，葡萄糖阻遏在功能性线粒体膜的合成中起重要作用。
