噬菌体T4感染后与大肠杆菌DNA依赖性RNA聚合酶相关的新型小多肽。
New small polypeptides associated with DNA-dependent RNA polymerase of Escherichia coli after infection with bacteriophage T4.
作者信息
Stevens A
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):603-7. doi: 10.1073/pnas.69.3.603.
Abstract
Four new small polypeptides are associated with DNA-dependent RNA polymerase from E. coli after infection with T4 phage. The new polypeptides are easily detected in RNA polymerase from E. coli cells labeled with amino acids after phage infection. Their molecular weights range from 10,000 to 22,000, as detected by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. All four polypeptides are found after infection with either wild-type T4 phage or T4 early amber mutants in genes 44, 42, 47, and 46. None of the polypeptides is labeled significantly before 5 min after infection at 30 degrees . When two maturation-defective amber mutants in gene 55 of T4 phage are used for infection, a polypeptide with a molecular weight of 22,000 is absent. When a maturation-defective amber mutant in gene 33 of T4 phage is used, another small protein is absent.
摘要
用T4噬菌体感染大肠杆菌后，四种新的小多肽与大肠杆菌的DNA依赖性RNA聚合酶相关联。在噬菌体感染后用氨基酸标记的大肠杆菌细胞的RNA聚合酶中很容易检测到这些新多肽。通过在十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳检测，它们的分子量范围为10,000至22,000。用野生型T4噬菌体或基因44、42、47和46中的T4早期琥珀突变体感染后均能发现这四种多肽。在30摄氏度感染后5分钟之前，没有一种多肽被显著标记。当使用T4噬菌体基因55中的两个成熟缺陷型琥珀突变体进行感染时，分子量为22,000的多肽不存在。当使用T4噬菌体基因33中的成熟缺陷型琥珀突变体时，另一种小蛋白质不存在。
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