成年犬心丝虫（恶丝虫）的己糖激酶
Hexokinase of the adult dog heartworm, Dirofilaria immitis.
作者信息
Hutchison W F, Turner A C, Oelshlegel F J
机构信息
Department of Preventive Medicine, University of Mississippi School of Medicine, Jackson 39216.
出版信息
Comp Biochem Physiol B. 1977;58(2):131-4. doi: 10.1016/0305-0491(77)90097-9.
PMID:45524
Abstract
- Dirofilaria immitis hexokinase was relatively heat stable and had a pH optimum range between 7.8 and 8.2. 2. Mean Vmax was 0.40 +/- 0.10 (S.D.) mumole/min/100 mg of worm. 3. Mean Km values were 0.32 mM for glucose, 0.86 mM for fructose and 0.39 mM for ATP. 4. Glucose-6-phosphate was not a strong product inhibitor. 5. Starch gel electrophoresis demonstrated at least three isozymes.
摘要
- 犬恶丝虫己糖激酶相对耐热，最适pH范围在7.8至8.2之间。2. 平均最大反应速度（Vmax）为0.40±0.10（标准差）微摩尔/分钟/100毫克虫体。3. 葡萄糖的平均米氏常数（Km）值为0.32毫摩尔，果糖为0.86毫摩尔，三磷酸腺苷（ATP）为0.39毫摩尔。4. 6-磷酸葡萄糖不是强产物抑制剂。5. 淀粉凝胶电泳显示至少有三种同工酶。
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