通过碳-13核磁共振光谱法测定致龋生物变形链球菌的酶合成葡聚糖的组成。

Determination, by carbon-13 nuclear magnetic resonance spectroscopy, of the composition of glucans synthesized by enzymes of the cariogenic organism Streptococcus mutans.

作者信息

Colson P, Jarrell H C, Lamberts B L, Smith I C

出版信息

Carbohydr Res. 1979 Jun;71:265-72. doi: 10.1016/s0008-6215(00)86074-7.

Abstract

The 13C-n.m.r. spectra of water-soluble and -insoluble glucans synthesized by enzymes isolated from six strains of Streptococcus mutans are interpreted. The glucans are shown to be composed primarily of alpha-(1 linked to 3)- and alpha-(1 linked to 6)-linked glucosyl residues, and the relative abundance of each linkage is estimated from peak areas. Treatment of water-insoluble glucans with dextranase is found to result in water-soluble and -insoluble products, the former enriched in alpha-(1 linked to 6)-linkages and the latter in alpha-(1 linked to 3)-linkages. The structural conclusions arrived at by 13C-n.m.r. spectroscopy are consistent with data from methylation analysis and 1H-n.m.r. spectroscopy.

摘要

对从六株变形链球菌中分离出的酶所合成的水溶性和水不溶性葡聚糖的碳-13核磁共振光谱进行了解读。结果表明，这些葡聚糖主要由α-(1→3)和α-(1→6)连接的葡萄糖残基组成，并且根据峰面积估算了每种连接的相对丰度。发现用水解葡聚糖酶处理水不溶性葡聚糖会产生水溶性和水不溶性产物，前者富含α-(1→6)连接，后者富含α-(1→3)连接。通过碳-13核磁共振光谱得出的结构结论与甲基化分析和氢-1核磁共振光谱的数据一致。