帝王蟹血蓝蛋白氧亲和力的自动测量

Automatic measurement of the oxygen affinity of Cancer magister hemocyanin.

Wajcman H, McMahill P, Mason H S

Department of Biochemistry, School of Medicine, University of Oregon, Health Sciences Center, Portland 97201.

Comp Biochem Physiol B. 1977;57(2):139-41. doi: 10.1016/0305-0491(77)90163-8.

Abstract

The P50 of Cancer Magister hemocyanin in hemolymph was 40.0 mm at 25 degrees C, nmax = 3.9; at 14 degrees C, 15.0 mm and 3.4; and at 10 degrees C. 10.2 mm and 3.3. 2. In the absence of Ca2+ and Mg2+, the P50 at 25 degrees C was in the range of 150 to 250 mm, depending upon pH. 3. Our results show that temperature variation and pH variation may be important factors in the physiological regulation of oxygen delivery in this species. 4. The automatic recording device of Imai et al. (1970) previously used for the study of hemoglobins, was found to be eminently suitable for the study of O2 affinities of hemocyanins.

摘要

在25摄氏度时，海蟹血蓝蛋白在血淋巴中的P50为40.0毫米，nmax = 3.9；在14摄氏度时，分别为15.0毫米和3.4；在10摄氏度时，分别为10.2毫米和3.3。2. 在没有Ca2+和Mg2+的情况下，25摄氏度时的P50在150至250毫米范围内，具体取决于pH值。3. 我们的结果表明，温度变化和pH值变化可能是该物种氧气输送生理调节中的重要因素。4. 发现Imai等人（1970年）先前用于血红蛋白研究的自动记录装置非常适合用于血蓝蛋白的氧亲和力研究。