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核酮糖-1,5-二磷酸羧化酶小亚基编码基因的重复频率

Reiteration frequency of the gene coding for the small subunit of ribulose--1,5--bisphosphate carboxylase.

作者信息

Cashmore A R

出版信息

Cell. 1979 Jun;17(2):383-8. doi: 10.1016/0092-8674(79)90164-8.

DOI:10.1016/0092-8674(79)90164-8
PMID:455470
Abstract

The messenger RNA coding for the precursor of the small subunit of ribulose--1,5--bisphosphate (Ru-P2) carboxylase has been partially purified from pea leaves. The mRNA has a size of 11.5S, is approximately 800 nucleotides long and, on cell-free translation, directs the synthesis of a single major polypeptide of 20,000 daltons. Antiserum for the small subunit of Ru-P2 carboxylase immunoprecipitated 52% of the 35S--methionine-labeled cell-free translation products. The RNA hybridizes with pea DNA with monophasic kinetics corresponding to the presence of one, or very few, gene copies per haploid genome.

摘要

编码核酮糖-1,5-二磷酸羧化酶小亚基前体的信使核糖核酸已从豌豆叶片中部分纯化出来。该信使核糖核酸大小为11.5S，约800个核苷酸长，在无细胞翻译体系中，可指导合成一条分子量为20,000道尔顿的主要多肽。核酮糖-1,5-二磷酸羧化酶小亚基的抗血清免疫沉淀了35S-甲硫氨酸标记的无细胞翻译产物的52%。该核糖核酸与豌豆脱氧核糖核酸杂交，呈现单相动力学，这表明单倍体基因组中存在一个或极少数基因拷贝。

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Reiteration frequency of the gene coding for the small subunit of ribulose--1,5--bisphosphate carboxylase.核酮糖-1,5-二磷酸羧化酶小亚基编码基因的重复频率
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2
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引用本文的文献

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Plant Mol Biol. 1986 Jan;6(1):13-21. doi: 10.1007/BF00021302.
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Expression of nuclear and plastid genes for photosynthesis-specific proteins during tomato fruit development and ripening.在番茄果实发育和成熟过程中，光合作用相关蛋白的核基因和质体基因的表达。
Plant Mol Biol. 1986 Sep;7(5):367-76. doi: 10.1007/BF00032566.
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Photooxidative destruction of chloroplasts and its consequences for expression of nuclear genes.
叶绿体的光氧化破坏及其对核基因表达的影响。
Planta. 1986 Jan;167(1):106-13. doi: 10.1007/BF00446376.
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Curr Genet. 1983 Apr;7(2):129-38. doi: 10.1007/BF00365638.
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