Lin P S, Chen H W
Cell Biol Int Rep. 1979 Jan;3(1):51-9. doi: 10.1016/0309-1651(79)90068-7.
The effect of sterol depletion on the topology of mouse L cells was studied by scanning electron microscopy. Treatment of cells with 25-hydroxycholesterol inhibited the synthesis of cellular sterol and diminished the number of microvilli on the cell surface. Simultaneous addition of mevalonic acid or cholesterol to cells during treatment with the inhibitor prevented the loss of microvilli. These results demonstrate that cholesterol is important in maintaining the ultrastructure of the surface membrane of nucleated mammalian cells.
通过扫描电子显微镜研究了甾醇耗竭对小鼠L细胞拓扑结构的影响。用25-羟基胆固醇处理细胞可抑制细胞甾醇的合成，并减少细胞表面微绒毛的数量。在用抑制剂处理细胞期间，同时向细胞中添加甲羟戊酸或胆固醇可防止微绒毛的丢失。这些结果表明，胆固醇在维持有核哺乳动物细胞表面膜的超微结构中很重要。
