黑腹果蝇δ1-吡咯啉-5-羧酸还原酶的特性
Characteristics of delta 1-pyrroline-5-carboxylate reductase from Drosophila melanogaster.
作者信息
Farmer J L, Bradshaw W S, Smith C S
机构信息
Department of Zoology, Brigham Young University, Provo, UT.
出版信息
Comp Biochem Physiol B. 1979;62(2):143-6. doi: 10.1016/0305-0491(79)90300-6.
PMID:45549
Abstract
- Biochemical properties of delta 1-pyrroline-5-carboxylate reductase from d. melanogaster have been investigated. 2. The enzyme is stable below 4 degrees C. 3. the pH optimum of the enzyme is 5.7. It is rapidly inactivated below pH 5.4. 4. The Km values for NADPH and delta 1-pyrroline-5-carboxylate are 1.6 x 10-5 and 2.5 x 10-6 M, respectively. 5. the estimated molecular weight of the enzyme is 225,000. 6. the enzyme is weakly inhibited by L-proline (Ki = 0.12 M).
摘要
- 对黑腹果蝇的δ1-吡咯啉-5-羧酸还原酶的生化特性进行了研究。2. 该酶在4摄氏度以下稳定。3. 该酶的最适pH值为5.7。在pH 5.4以下会迅速失活。4. NADPH和δ1-吡咯啉-5-羧酸的Km值分别为1.6×10⁻⁵和2.5×10⁻⁶ M。5. 该酶的估计分子量为225,000。6. 该酶受到L-脯氨酸的微弱抑制（Ki = 0.12 M）。
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