Lameijer L D, Smith J, Mowbray J F
Br J Exp Pathol. 1972 Apr;53(2):130-7.
A simple method of orthotopic renal transplantation in the rat is briefly described. With this method the ability of azathioprine to prolong the survival of renal transplants in rats has been studied. Measurement of the amount of blood contained in a transplanted kidney has been made as this is the major source of passenger cells which can immunize the recipient. The efficacy of removal of blood by perfusion of the kidney before transplantation with its effect on the efficiency of azathioprine in preventing rejection has been studied. Using a standard 12 mg/kg daily dose of azathioprine, survival and histological damage to the graft is shown to be related to the amount of blood present in the kidney at the time of transplantation. Rat renal allograft rejection may be used as an assay system of the effect of immunosuppressive drugs so long as the same procedure is used throughout the experiment.
简要描述了一种大鼠原位肾移植的简单方法。采用这种方法研究了硫唑嘌呤延长大鼠肾移植存活时间的能力。已对移植肾所含血量进行了测量，因为这是可使受体产生免疫反应的过客细胞的主要来源。研究了移植前通过灌注肾脏去除血液的效果及其对硫唑嘌呤预防排斥反应效率的影响。使用每日12 mg/kg的标准硫唑嘌呤剂量时，移植物的存活和组织学损伤与移植时肾脏中的血量有关。只要在整个实验中采用相同的程序，大鼠肾同种异体移植排斥反应就可作为免疫抑制药物效果的检测系统。
