Amemiya H, Yokoyama T, Putnam C W, Torisu M, Starzl T E
Clin Exp Immunol. 1972 Mar;10(3):417-26.
ALG raised against lymphoblasts grown in pure culture for many generations contained antiplatelet activity. The thromboagglutinins could be completely removed by absorption with lymphoblasts, indicating that they had been raised to antigens shared by lymphoblasts and platelets. Anti-spleen ALG possessed levels of such anti-platelet antibodies but in substantially higher titres, because an additional contribution was made by contaminating thrombocytes in the immunizing injectate. By chromatographic separation and immunoelectrophoretic analysis of the eluate from platelet–antibody complexes generated during the absorption of anti-spleen ALG with thrombocytes, the thromboagglutinins were shown to reside almost exclusively (97·7%) in the area of the IgA fraction. The direct Coombs' test, reacting platelets from patients receiving equine anti-spleen ALG with guinea-pig antisera against normal horse serum, showed a highly significant, but not invariable correlation with clinical platelet depression, which was not, however, clearly related to the thromboagglutinin titre of the ALG being administered.
针对在纯培养中生长多代的淋巴母细胞产生的抗淋巴细胞球蛋白（ALG）具有抗血小板活性。血栓凝集素可通过与淋巴母细胞吸附而完全去除，这表明它们是针对淋巴母细胞和血小板共有的抗原产生的。抗脾脏ALG具有此类抗血小板抗体，但效价显著更高，因为免疫注射物中污染的血小板也有额外作用。通过对用血小板吸收抗脾脏ALG过程中产生的血小板 - 抗体复合物洗脱液进行色谱分离和免疫电泳分析，发现血栓凝集素几乎完全（97.7%）存在于IgA组分区域。直接库姆斯试验中，用豚鼠抗正常马血清抗血清与接受马抗脾脏ALG治疗患者的血小板反应，结果显示与临床血小板减少有高度显著但并非恒定的相关性，然而，这与所给予的ALG的血栓凝集素效价并无明确关联。
