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1
Maturation of the immkune response in vitro. Focal fluctuation and changes in affinity of anti-beta-D-galactosidase activating antibody.体外免疫反应的成熟。抗β-D-半乳糖苷酶激活抗体的局灶性波动及亲和力变化。
J Exp Med. 1972 Aug 1;136(2):353-68. doi: 10.1084/jem.136.2.353.
2
In vitro antibody response to a distinct antigenic determinant of Escherichia coli beta-D-galactosidase. Effect of antigen dosage and co-cultivation of lymph node fragments on peak titres and duration of antibody synthesis.对大肠杆菌β-D-半乳糖苷酶一个独特抗原决定簇的体外抗体应答。抗原剂量及淋巴结片段共培养对抗体合成峰值滴度和持续时间的影响。
Immunology. 1974 Jul;27(1):43-52.
3
Association constant and heterogeneity index of antibodies specific for a natural determinant of a bacterial protein. Time-course studies during the in vitro immune response to a distinct site of Escherichia coli beta-D-galactosidase.针对细菌蛋白天然决定簇的抗体的结合常数和异质性指数。对大肠杆菌β-D-半乳糖苷酶不同位点的体外免疫应答过程中的时间进程研究。
J Immunol. 1974 Sep;113(3):756-63.
4
An immunoenzymatic system to study in vitro immune responses. Synthesis of antibodies directed toward different -D-galactosidase determinants in lymph node fragment cultures.一种用于研究体外免疫反应的免疫酶系统。在淋巴结片段培养物中合成针对不同β-D-半乳糖苷酶决定簇的抗体。
Immunology. 1973 Feb;24(2):237-48.
5
Enzyme activation by antibodies: a method to determine the binding constant of the activating antibody towards one determinant of E. coli beta-D-galactosidase.抗体介导的酶激活：一种测定激活抗体与大肠杆菌β-D-半乳糖苷酶一个决定簇结合常数的方法。
Immunochemistry. 1973 Dec;10(12):797-804. doi: 10.1016/0019-2791(73)90183-3.
6
Long-term antibody synthesis in vitro. VI. Anti-allotype sera as probes of clonal products in affinity maturation.体外长期抗体合成。VI. 抗同种异型血清作为亲和力成熟中克隆产物的探针。
Immunology. 1978 Aug;35(2):341-52.
7
[Asynchronism in the synthesis of antibodies directed against different antigenic sites of a macromolecule].[针对大分子不同抗原位点的抗体合成中的异步现象]
Ann Immunol (Paris). 1977 Jan-Mar;128(1-2):173-5.
8
Long-lasting in vitro immune response to a distinct antigenic determinant of a bacterial protein. Cyclic changes of antibody titer and affinity.对细菌蛋白独特抗原决定簇的持久体外免疫反应。抗体滴度和亲和力的周期性变化。
J Immunol. 1975 Jul;115(1):106-11.
9
Long-term antibody synthesis in vitro- IV. Independent segregation of antibodies directed to different determinants of an antigen molecule in its native configuration.体外长期抗体合成 - 四、针对天然构象抗原分子不同决定簇的抗体的独立分离
Immunology. 1976 Oct;31(4):553-61.
10
Interference of anti-allotype antisera with antigen-antibody binding.抗独特型抗血清对抗原-抗体结合的干扰。
J Immunol. 1975 Apr;114(4):1430-3.

引用本文的文献

1
In vitro antibody response to a distinct antigenic determinant of Escherichia coli beta-D-galactosidase. Effect of antigen dosage and co-cultivation of lymph node fragments on peak titres and duration of antibody synthesis.对大肠杆菌β-D-半乳糖苷酶一个独特抗原决定簇的体外抗体应答。抗原剂量及淋巴结片段共培养对抗体合成峰值滴度和持续时间的影响。
Immunology. 1974 Jul;27(1):43-52.
2
An immunoenzymatic system to study in vitro immune responses. Synthesis of antibodies directed toward different -D-galactosidase determinants in lymph node fragment cultures.一种用于研究体外免疫反应的免疫酶系统。在淋巴结片段培养物中合成针对不同β-D-半乳糖苷酶决定簇的抗体。
Immunology. 1973 Feb;24(2):237-48.
3
Long-term antibody synthesis in vitro. VI. Anti-allotype sera as probes of clonal products in affinity maturation.体外长期抗体合成。VI. 抗同种异型血清作为亲和力成熟中克隆产物的探针。
Immunology. 1978 Aug;35(2):341-52.
4
Long-term antibody synthesis in vitro- IV. Independent segregation of antibodies directed to different determinants of an antigen molecule in its native configuration.体外长期抗体合成 - 四、针对天然构象抗原分子不同决定簇的抗体的独立分离
Immunology. 1976 Oct;31(4):553-61.
5
Antisera against electrophoretically purified tubulin stimulate colchicine-binding activity.针对经电泳纯化的微管蛋白的抗血清可刺激秋水仙碱结合活性。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1246-9. doi: 10.1073/pnas.73.4.1246.

本文引用的文献

1
Antibody-mediated activation of a defective beta-D-galactosidase. II. Immunological relationship between the normal and the defective enzyme.抗体介导的缺陷型β-D-半乳糖苷酶的激活。II. 正常酶与缺陷酶之间的免疫学关系。
J Exp Med. 1971 Sep 1;134(3 Pt 1):751-64. doi: 10.1084/jem.134.3.751.
2
VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE.免疫应答过程中抗体亲和力的变化
Biochemistry. 1964 Jul;3:996-1008. doi: 10.1021/bi00895a027.
3
Studies on antibody production. V. The secondary response in vitro.抗体产生的研究。V. 体外二次应答。
J Exp Med. 1963 Jun 1;117(6):1035-51. doi: 10.1084/jem.117.6.1035.
4
Determination of avidity of anti-albumin antibodies in the mouse. Influence of the number of cells transferred on the quality of the secondary adoptive response.小鼠抗白蛋白抗体亲和力的测定。转移细胞数量对二次过继性反应质量的影响。
Immunology. 1969 Aug;17(2):189-98.
5
Symposium on in vitro studies of the immune response. I. Variations in the immune response to a simple determinant.免疫反应的体外研究研讨会。I. 对单一决定簇免疫反应的变化
Bacteriol Rev. 1966 Jun;30(2):383-96. doi: 10.1128/br.30.2.383-396.1966.
6
Studies on the regulation of avidity at the level of the single antibody-forming cell. The effect of antigen dose and time after immunization.关于单个抗体形成细胞水平亲和力调节的研究。抗原剂量和免疫后时间的影响。
J Exp Med. 1970 Jul 1;132(1):77-88. doi: 10.1084/jem.132.1.77.
7
Antibody-mediated activation of a defective beta-D-galactosidase extracted from an Escherichia coli mutant.从大肠杆菌突变体中提取的缺陷型β-D-半乳糖苷酶的抗体介导激活作用。
Proc Natl Acad Sci U S A. 1968 Jun;60(2):660-7. doi: 10.1073/pnas.60.2.660.
8
The effect of antigen dose and time after immunization on the amount and affinity of anti-hapten antibody.抗原剂量及免疫后时间对抗半抗原抗体数量和亲和力的影响。
J Immunol. 1968 Feb;100(2):371-5.
9
Secondary antibody responses in haptenic systems: cell population selection by antigen.半抗原系统中的二次抗体应答：抗原对细胞群体的选择
J Immunol. 1967 Oct;99(4):760-70.
10
The relative affinity of antibodies synthesized in the secondary response.二次免疫应答中合成的抗体的相对亲和力。
J Exp Med. 1967 Dec 1;126(6):1185-205. doi: 10.1084/jem.126.6.1185.

体外免疫反应的成熟。抗β-D-半乳糖苷酶激活抗体的局灶性波动及亲和力变化。

Maturation of the immkune response in vitro. Focal fluctuation and changes in affinity of anti-beta-D-galactosidase activating antibody.

作者信息

Macario A J, Conway de Macario E, Franceschi C, Celada F

出版信息

J Exp Med. 1972 Aug 1;136(2):353-68. doi: 10.1084/jem.136.2.353.

DOI:10.1084/jem.136.2.353
PMID:4557772
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2139213/
Abstract

We have cultivated lymph node microfragments from beta-D-galactosidase (Escherichia coli) primed rabbits and have measured their secondary response directed towards the whole molecule (precipitating antibodies) and to a single determinant (activating antibodies) of the antigen. By decreasing the size of the fragments to 10(5) cells, we began to observe heterogeneity among identical cultures in terms of positivity of response, antibody specificity, and titers. The affinity of "early" activating antibodies was inversely proportional to the dose of challenge. While no maturation was seen in low and excessive challenge, in all cultures receiving intermediate doses the association constant was raised several orders of magnitude within periods of 20 days. The relevance of these data to the mechanism of affinity selection of antigen-sensitive cells is discussed.

摘要

我们从用β-D-半乳糖苷酶（大肠杆菌）致敏的兔子身上培养了淋巴结微片段，并测定了它们针对抗原的整个分子（沉淀抗体）和单个决定簇（激活抗体）的二次反应。通过将片段大小减小到10⁵个细胞，我们开始观察到相同培养物之间在反应阳性、抗体特异性和滴度方面的异质性。“早期”激活抗体的亲和力与攻击剂量成反比。在低剂量和高剂量攻击时未观察到成熟现象，而在所有接受中等剂量攻击的培养物中，结合常数在20天内提高了几个数量级。讨论了这些数据与抗原敏感细胞亲和力选择机制的相关性。