Andersson B
J Exp Med. 1970 Jul 1;132(1):77-88. doi: 10.1084/jem.132.1.77.
The hemolytic plaque formation of cells producing antibody against heterologous albumins was tested for sensitivity to specific inhibition by free antigen. The inhibition characteristics of plaques in this system were found to be a measure for the avidity of the antibody produced by the plaque-forming cells (PFC:s). High avidity-producing PFC:s were more sensitive to inhibition than low avidity PFC:s. Immunization with a high dose of antigen induced PFC:s that produced antibody with a lower avidity as compared to PFC:s from animals immunized with a low dose. The avidity was increased with time. Determinations of avidity at the serum level were also made, and the results were in agreement with the findings at the cellular level. The present method made it possible to demonstrate differences in avidity of antibody at the level of the single antibody-forming cell. It may also constitute a useful tool for the analysis of the cellular events leading to the production of antibodies with varying affinities during the immune response.
对产生抗异源白蛋白抗体的细胞进行溶血空斑形成试验，以检测其对游离抗原特异性抑制的敏感性。发现该系统中空斑的抑制特性是对空斑形成细胞（PFC）产生的抗体亲和力的一种度量。产生高亲和力的PFC比产生低亲和力的PFC对抑制更敏感。用高剂量抗原免疫诱导产生的PFC所产生的抗体，其亲和力低于用低剂量抗原免疫的动物所产生的PFC。亲和力随时间增加。还进行了血清水平亲和力的测定，结果与细胞水平的发现一致。本方法能够在单个抗体形成细胞水平上证明抗体亲和力的差异。它也可能构成一种有用的工具，用于分析免疫反应过程中导致产生具有不同亲和力抗体的细胞事件。
