The immunogenic components of equine anti-human lymphocyte lobulin in renal allograft recipients.

The antigenic components responsible for anti-ALG antibodies in the sera of ten human renal allograft recipients during ALG treatment have been evaluated. By sequential anion and cation exchange column chromatography, four ALG subfractions were demonstrated. In all cases, the antibodies were directed against the anodic components with relatively high hexose content. No precipitating antibodies were detected against the cathodic subfractions with low hexose content. Thus, the production and clinical use of pure cathodic γG-fractions of low antigenicity seems warranted.