Sharpe A N, Biggs D R, Oliver R J
Appl Microbiol. 1972 Jul;24(1):70-6. doi: 10.1128/am.24.1.70-76.1972.
A fully automatic system for preparing poured plates for bacteriological analyses has been constructed and tested. The machine can make decimal dilutions of bacterial suspensions, dispense measured amounts into petri dishes, add molten agar, mix the dish contents, and label the dishes with sample and dilution numbers at the rate of 2,000 dishes per 8-hr day. In addition, the machine can be programmed to select different media so that plates for different types of bacteriological analysis may be made automatically from the same sample. The machine uses only the components of the media and sterile polystyrene petri dishes; requirements for all other materials, such as sterile pipettes and capped bottles of diluents and agar, are eliminated.
已构建并测试了一种用于制备用于细菌学分析的倾注平板的全自动系统。该机器可以对细菌悬液进行十倍稀释,将测量好的量分配到培养皿中,加入融化的琼脂,混合培养皿中的内容物,并以每天8小时制备2000个培养皿的速度用样品和稀释倍数标记培养皿。此外,该机器可以编程选择不同的培养基,以便可以从同一样品中自动制备用于不同类型细菌学分析的平板。该机器仅使用培养基的成分和无菌聚苯乙烯培养皿;消除了对所有其他材料的需求,如无菌移液管和带帽的稀释剂及琼脂瓶。