T4噬菌体特异性酶的体外DNA指导合成。
DNA-directed synthesis in vitro of T4 phage-specific enzymes.
作者信息
Natale P J, Buchanan J M
出版信息
Proc Natl Acad Sci U S A. 1972 Sep;69(9):2513-7. doi: 10.1073/pnas.69.9.2513.
Abstract
The synthesis of deoxynucleotide kinase (EC 2.7.4.2) in vitro by a preparation consisting of T4 bacteriophage DNA and a cell-free extract of Escherichia coli has been reported. A study of the role of monovalent cations in the synthesis of this enzyme as well as alpha-glucosyl transferase (EC 2.4.1.2) shows that potassium ions are required for maximal enzyme production. Examination of the RNA-directed system indicates that potassium ions are more effective than ammonium ion in the translation of messenger RNA for the formation of the biologically active proteins studied. From a comparison of the magnitude of the effect of ions on both the DNA- and RNA-directed systems, we conclude that potassium ions may also have a marked stimulatory effect on transcription of the deoxynucleotide kinase gene of T4 DNA. The time required for messenger initiation and completion and the size distribution of messenger RNA formed in vitro were also examined.
摘要
据报道，由T4噬菌体DNA和大肠杆菌无细胞提取物组成的制剂可在体外合成脱氧核苷酸激酶（EC 2.7.4.2）。对单价阳离子在该酶以及α-葡糖基转移酶（EC 2.4.1.2）合成中的作用进行的研究表明，钾离子是最大量产生酶所必需的。对RNA指导系统的研究表明，在用于形成所研究的生物活性蛋白质的信使RNA的翻译过程中，钾离子比铵离子更有效。通过比较离子对DNA指导和RNA指导系统的影响程度，我们得出结论，钾离子可能对T4 DNA的脱氧核苷酸激酶基因的转录也有显著的刺激作用。还研究了信使起始和完成所需的时间以及体外形成的信使RNA的大小分布。
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