由噬菌体“T4-RNA”编程的脱氧核苷酸激酶的体外合成
In vitro synthesis of deoxynucleotide kinase programmed by bacteriophage "T4-RNA.
作者信息
Sakiyama S, Buchanan J M
出版信息
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1376-80. doi: 10.1073/pnas.68.6.1376.
Abstract
RNA extracted from T4 phage-infected Escherichia coli cells can direct the synthesis of phage-specific deoxynucleotide kinase in a cell-free system from uninfected E. coli. The maximum messenger activity is obtained 8 min after infection at 37 degrees C and decreases there-after. The main activity of this messenger RNA has a sedimentation coefficient of about 15 S on a sucrose density gradient. The conditions necessary for the appearance of enzyme activity in vitro are the same as those required for de novo synthesis of protein. RNA from cells infected with a T4 amber mutant incapable of inducing the kinase in vivo lacks the ability to direct the synthesis of enzyme in vitro.
摘要
从T4噬菌体感染的大肠杆菌细胞中提取的RNA，能够在未感染的大肠杆菌的无细胞体系中指导噬菌体特异性脱氧核苷酸激酶的合成。在37℃感染8分钟后可获得最大信使活性，之后活性降低。这种信使RNA的主要活性在蔗糖密度梯度上的沉降系数约为15S。体外出现酶活性所需的条件与蛋白质从头合成所需的条件相同。来自感染了T4琥珀突变体（该突变体在体内不能诱导激酶产生）的细胞的RNA，缺乏在体外指导酶合成的能力。
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