大肠杆菌K-12的细胞壁肽聚糖突变体：存在两个用于细胞壁肽聚糖生物合成的基因簇，即mra和mrb。
Cell wall peptidoglycan mutants of Escherichia coli K-12: existence of two clusters of genes, mra and mrb, for cell wall peptidoglycan biosynthesis.
作者信息
Miyakawa T, Matsuzawa H, Matsuhashi M, Sugino Y
出版信息
J Bacteriol. 1972 Nov;112(2):950-8. doi: 10.1128/jb.112.2.950-958.1972.
Abstract
Temperature-sensitive mutants of Escherichia coli K-12 which cannot form cell wall peptidoglycan at 42 C were isolated. The thermosensitive steps were characterized biochemically, and the genes coding the enzymes of peptidoglycan synthesis were mapped. These genes were in two clusters: one group, located at about 1.5 min between leu and azi, was designated as mra (murein a); the second group, located at about 77.5 min close to argH and metB, was designated as mrb (murein b, with the order metB-argH-mrb). No simple relations were found between the gene location and the order or localization of enzymes involved in the sequence of reactions of cell wall peptidoglycan biosynthesis.
摘要
分离出了大肠杆菌K - 12的温度敏感突变体，这些突变体在42℃时不能形成细胞壁肽聚糖。对温度敏感步骤进行了生化表征，并对编码肽聚糖合成酶的基因进行了定位。这些基因分为两个簇：一组位于亮氨酸（leu）和叠氮化物抗性基因（azi）之间约1.5分钟处，被命名为mra（胞壁质a）；第二组位于靠近精氨酸琥珀酸合成酶基因（argH）和蛋氨酸合成酶基因（metB）约77.5分钟处，被命名为mrb（胞壁质b，顺序为metB - argH - mrb）。在细胞壁肽聚糖生物合成反应序列中涉及的酶的基因位置与顺序或定位之间未发现简单的关系。
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本文引用的文献
1
Replica plating and indirect selection of bacterial mutants.细菌突变体的影印培养和间接筛选
J Bacteriol. 1952 Mar;63(3):399-406. doi: 10.1128/jb.63.3.399-406.1952.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE GENETIC MAP OF ESCHERICHIA COLI K-12.大肠杆菌K-12的遗传图谱
Genetics. 1964 Oct;50(4):659-77. doi: 10.1093/genetics/50.4.659.
4
BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS.袋状大分子——对细菌细胞壁的新视角
Adv Enzymol Relat Subj Biochem. 1964;26:193-232. doi: 10.1002/9780470122716.ch5.
5
Transduction of linked genetic characters of the host by bacteriophage P1.噬菌体P1对宿主连锁遗传性状的转导
Virology. 1955 Jul;1(2):190-206. doi: 10.1016/0042-6822(55)90016-7.
6
Biosythesis of the peptidoglycan of bacterial cell walls. II. Phospholipid carriers in the reaction sequence.细菌细胞壁肽聚糖的生物合成。II. 反应序列中的磷脂载体。
J Biol Chem. 1967 Jul 10;242(13):3180-90.
7
A simple procedure for the preparation of UDP-N-acetylglucosamine-14C.一种制备UDP-N-乙酰葡糖胺-14C的简单方法。
Biochem Biophys Res Commun. 1970 May 22;39(4):718-22. doi: 10.1016/0006-291x(70)90264-0.
8
Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli.大肠杆菌中D-环丝氨酸抑制作用及抗性的动力学和遗传学分析
J Bacteriol. 1965 Nov;90(5):1238-50. doi: 10.1128/jb.90.5.1238-1250.1965.
9
Peptidoglycan transpeptidase and D-alanine carboxypeptidase: penicillin-sensitive enzymatic reactions.肽聚糖转肽酶和D-丙氨酸羧肽酶：青霉素敏感的酶促反应。
Fed Proc. 1967 Jan-Feb;26(1):9-22.
10
Role of lipopolysaccharides in antibiotic resistance and bacteriophage adsorption of Escherichia coli K-12.脂多糖在大肠杆菌K-12抗生素抗性及噬菌体吸附中的作用
J Bacteriol. 1971 Mar;105(3):968-75. doi: 10.1128/jb.105.3.968-975.1971.
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