大肠杆菌限制性内切酶K和P的DNA修饰甲基化酶活性
DNA modification methylase activity of Escherichia coli restriction endonucleases K and P.
作者信息
Haberman A, Heywood J, Meselson M
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3138-41. doi: 10.1073/pnas.69.11.3138.
Abstract
The highly purified restriction endonucleases of E. coli K and coliphage P1 transfer methyl groups from S-adenosylmethionine to adenine residues of unmodified DNA. Incubation of unmodified DNA with endonucleases K or P and S-adenosylmethionine renders the DNA resistant to restriction. The enzymes, therefore, have both restriction endonuclease and modification methylase activities.
摘要
大肠杆菌K和噬菌体P1的高度纯化的限制性内切酶可将甲基基团从S-腺苷甲硫氨酸转移至未修饰DNA的腺嘌呤残基上。将未修饰的DNA与内切酶K或P以及S-腺苷甲硫氨酸一起温育会使DNA具有抗限制性。因此，这些酶同时具有限制性内切酶和修饰甲基化酶的活性。
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本文引用的文献
1
Structure of the DNA of bacteriophage phiX174. VII. Methylation.噬菌体φX174的DNA结构。VII. 甲基化
J Mol Biol. 1970 Oct 28;53(2):251-9. doi: 10.1016/0022-2836(70)90298-6.
2
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.一种来自流感嗜血杆菌的限制性内切酶。I. 纯化及一般特性。
J Mol Biol. 1970 Jul 28;51(2):379-91. doi: 10.1016/0022-2836(70)90149-x.
3
The deoxyribonucleic acid modification enzyme of bacteriophage P1.噬菌体P1的脱氧核糖核酸修饰酶
Biochem J. 1972 Mar;127(1):1-10. doi: 10.1042/bj1270001.
4
Functional analysis of host-specificity mutants in Escherichia coli.大肠杆菌宿主特异性突变体的功能分析。
Genet Res. 1970 Apr;15(2):237-50. doi: 10.1017/s0016672300001567.
5
The Escherichia coli B restriction endonuclease.大肠杆菌B限制性内切酶
Biochim Biophys Acta. 1969 Nov 19;195(1):219-29. doi: 10.1016/0005-2787(69)90618-2.
6
DNA modification and restriction.DNA修饰与限制
Annu Rev Biochem. 1969;38:467-500. doi: 10.1146/annurev.bi.38.070169.002343.
7
A complementation analysis of the restriction and modification of DNA in Escherichia coli.大肠杆菌中DNA限制与修饰的互补分析。
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8
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Proc Natl Acad Sci U S A. 1969 Jun;63(2):556-62. doi: 10.1073/pnas.63.2.556.
9
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10
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Nature. 1968 Mar 23;217(5134):1110-4. doi: 10.1038/2171110a0.
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