Blasco L, Sokoloski J E, Wolf D P
Fertil Steril. 1979 Jul;32(1):55-60. doi: 10.1016/s0015-0282(16)44116-6.
Human sperm fertility potential, expressed as a quality index (QI), was evaluated objectively from considerations of sperm velocity, percentage of motile forms, sperm density, and ejaculate volume. Turbidimetry was applied in the quantitation of sperm velocity. High QIs (700) were characteristic of semen samples that were capable of penetrating cervical mucus in capillary tube penetration tests, while low QIs (80) were associated with specimens that did not penetrate mucus. The rate of decline in QI as a function of time postejaculation was determined for samples stored at 37 degrees C and at ambient temperature, providing correction factors for the comparative evaluation of semen samples analyzed at different times. The penetrability of cervical mucus was determined by capillary tube penetration testing. Subsequent chemical analysis of sperm-penetrable and impenetrable samples indicated that the concentrations of mucus nondialyzable solids (NDS), mucins, and soluble proteins were significantly higher in impenetrable specimens. These characteristics were also observed in impenetrable mucus obtained from patients with cervical factor infertility. These studies indicate that it is now feasible to employ mucus [NDS] in the diagnosis of cervical factor infertility and turbidimetry in the evaluation of human sperm quality.
从精子速度、活动形态百分比、精子密度和射精量等方面客观评估了以质量指数（QI）表示的人类精子生育潜力。采用比浊法对精子速度进行定量。高QI值（700）是精液样本在毛细管穿透试验中能够穿透宫颈黏液的特征，而低QI值（80）则与未穿透黏液的样本相关。确定了在37℃和环境温度下储存的样本中QI值随射精后时间的下降速率，为不同时间分析的精液样本的比较评估提供校正因子。通过毛细管穿透试验确定宫颈黏液的穿透性。对精子可穿透和不可穿透样本的后续化学分析表明，不可穿透样本中黏液不可透析固体（NDS）、黏蛋白和可溶性蛋白的浓度显著更高。在宫颈因素不孕症患者获得的不可穿透黏液中也观察到了这些特征。这些研究表明，现在采用黏液[NDS]诊断宫颈因素不孕症以及采用比浊法评估人类精子质量是可行的。
