λ噬菌体DNA：化学合成粘性末端的连接
Lambda phage DNA: joining of a chemically synthesized cohesive end.
作者信息
Harvey C L, Wright R, Nussbaum A L
出版信息
Science. 1973 Jan 19;179(4070):291-3. doi: 10.1126/science.179.4070.291.
PMID:4566655
Abstract
The chemically synthesized dodecamer d(pA-G-G-T-C-G-C-C-G-C-C-C) was annealed, and was covalently joined to lambda phage DNA with bacterio-phage T(4) ligase. The 5'-end of the dodecamer was joined to a deoxyguanosine residue. Repair with DNA polymerase I established that the position of joining was the left-hand end of lambda DNA. This is the first time that a chemically synthesized oligonucleotide has been covalently joined to a naturally occurring DNA molecule.
摘要
将化学合成的十二聚体d(pA-G-G-T-C-G-C-C-G-C-C-C)进行退火处理，并用噬菌体T(4)连接酶将其与λ噬菌体DNA共价连接。十二聚体的5'端与一个脱氧鸟苷残基相连。用DNA聚合酶I进行修复表明连接位置是λ DNA的左端。这是首次将化学合成的寡核苷酸与天然存在的DNA分子共价连接。
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