Zigmond S H, Hirsch J G
J Exp Med. 1973 Feb 1;137(2):387-410. doi: 10.1084/jem.137.2.387.
Polymorphonuclear leukocyte (PMN) locomotion and chemotaxis have been evaluated by direct microscopic observation of individual cells in thin slide-cover slip preparations, and also by observations on populations of cells migrating into a Millipore filter. The direct microscopic method used the polarity of the locomoting PMNs (broad, advancing lamellipodium and knoblike constriction at the rear) to record the direction of movement. The Boyden chamber Millipore assay was made more reliable by following the front of cells advancing into the filter, rather than counting the number of cells on the lower filter surface. Special modifications of the Millipore assay were necessary in order to distinguish between influences on rate of locomotion and true chemotaxis. In both systems the results indicate that under certain conditions leukocytes, and in particular PMNs, release into the medium a factor stimulating locomotion and exerting chemotactic action on PMNs in the vicinity. This cell-derived factor appears not to require serum factors for its release or action.
多形核白细胞（PMN）的运动和趋化性已通过在薄载玻片-盖玻片制剂中对单个细胞进行直接显微镜观察，以及对迁移到微孔滤膜中的细胞群体进行观察来评估。直接显微镜方法利用运动中的PMN的极性（宽阔的、向前的片状伪足和后方的瘤状收缩）来记录运动方向。通过跟踪进入滤膜的细胞前沿，而不是计算滤膜下表面的细胞数量，使博伊登室微孔试验更加可靠。为了区分对运动速率的影响和真正的趋化性，需要对微孔试验进行特殊修改。在这两种系统中，结果表明在某些条件下，白细胞，尤其是PMN，会向培养基中释放一种刺激运动并对附近的PMN发挥趋化作用的因子。这种细胞衍生因子的释放或作用似乎不需要血清因子。
