α-甘露糖苷酶与细胞黏菌苍白多囊黏菌中的微囊分化
Alpha-mannosidase and microcyst differentiation in the cellular slime mold Polysphondylium pallidum.
作者信息
O'Day D H
出版信息
J Bacteriol. 1973 Jan;113(1):192-7. doi: 10.1128/jb.113.1.192-197.1973.
The intracellular and extracellular pattern of alpha-mannosidase (EC 3.2.1.24) activity was studied during microcyst differentiation in the cellular slime mold, Polysphondylium pallidum. The evidence suggests that microcyst differentiation requires continuous protein synthesis. alpha-Mannosidase activity is present in amoebae and increases with differentiation, and the data indicate that this increase in activity requires concurrent protein synthesis. The enzyme is excreted during the differentiation process, and the release of the enzyme is not stopped by cycloheximide. A cystless mutant does not show the normal intracellular pattern of alpha-mannosidase but does excrete the enzyme. Microcyst differentiation is proposed as an alternative system to multicellular slime mold development for the biochemical analysis of certain aspects of cellular differentiation.
在细胞黏菌苍白聚孢霉的微孢囊分化过程中，研究了α-甘露糖苷酶（EC 3.2.1.24）活性的细胞内和细胞外模式。证据表明，微孢囊分化需要持续的蛋白质合成。α-甘露糖苷酶活性存在于变形虫中，并随着分化而增加，数据表明这种活性增加需要同时进行蛋白质合成。该酶在分化过程中被分泌出来，并且环己酰亚胺不会阻止该酶的释放。一个无孢囊突变体没有显示出正常的α-甘露糖苷酶细胞内模式，但确实会分泌该酶。微孢囊分化被提议作为多细胞黏菌发育的替代系统，用于细胞分化某些方面的生化分析。
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