Juurlink B H, Fedoroff S
In Vitro. 1979 Feb;15(2):86-94. doi: 10.1007/BF02618102.
Whole mouse embryos were grown in vitro from Theiler stage 12 (1 to 7 somites) to Theiler stages 15 and 16 (25 to 35 somites). This procedure gives experimental access to precisely staged embryos during the early period of neurogenesis. To follow the further development of neurons in vitro, fragments of spinal primordia were set up from these cultured embryos. In such cultures, the proliferation of precursor cells, the formation of postmitotic cells, and, finally, the cytodifferentiation of neurons were observed.
将整个小鼠胚胎在体外从第12期泰勒阶段（1至7体节）培养至第15和16期泰勒阶段（25至35体节）。该程序为在神经发生早期精确分期的胚胎提供了实验途径。为了追踪体外神经元的进一步发育，从这些培养的胚胎中分离出脊髓原基片段。在这样的培养物中，观察到前体细胞的增殖、有丝分裂后细胞的形成以及最终神经元的细胞分化。
