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φX174复制型DNA的两条链对电离辐射断裂的敏感性相同。

Equal sensitivity of the two strands of phiX174 replicative DNA to breakage by ionizing radiation.

作者信息

Achey P M, Courtney D G

出版信息

Biophys J. 1973 Jan;13(1):72-9. doi: 10.1016/s0006-3495(73)85970-3.

DOI:10.1016/s0006-3495(73)85970-3
PMID:4575520
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1484175/
Abstract

The supertwisted, double-stranded, replicative intermediate of øX174 DNA (RFI) has been used to determine whether one of the two strands of the double helix is uniquely sensitive to induction of single-strand breaks by ionizing radiation. This could result from a particularly sensitive base sequence or a transfer of energy to a specific location of the DNA molecule. The results indicate that both strands of the double helix are equally broken, even though their base compositions are significantly different. If there are "hot spots" in the strands, then they are present in equal amounts in each strand.

摘要

噬菌体φX174 DNA的超螺旋双链复制中间体（RFI）已被用于确定双螺旋的两条链中是否有一条对电离辐射诱导的单链断裂具有独特的敏感性。这可能是由于特定敏感的碱基序列，或者是能量转移到DNA分子的特定位置所致。结果表明，双螺旋的两条链被等量切断，尽管它们的碱基组成有显著差异。如果链中存在“热点”，那么每条链中的“热点”数量相等。

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本文引用的文献

1
The process of infection with bacteriophage phi-X174 VII. Ultracentrifugal analysis of the replicative form.噬菌体φ-X174的感染过程 VII. 复制型的超速离心分析
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The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.噬菌体φ-X174的感染过程。二十九。子代φ-X174噬菌体单链DNA合成的体内研究。
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3
Correlation of gamma-ray inactivation and strand scission in the replicative form of phi-X174 bacteriophage DNA.γ射线对φ-X174噬菌体DNA复制形式的失活作用与链断裂的相关性
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1753-7. doi: 10.1073/pnas.58.4.1753.
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Radiat Res. 1971 Dec;48(3):421-31.
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Polynucleotide ligase and phiX174 single strand synthesis.多核苷酸连接酶与φX174单链合成
Nat New Biol. 1971 Jun 9;231(23):170-3. doi: 10.1038/newbio231170a0.
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Structure of the replicative form of bacteriophage phi X174. V. Interconversions between twisted, extended and randomly coiled forms of cyclic DNA.噬菌体φX174复制型的结构。V. 环状DNA的扭曲、伸展和随机卷曲形式之间的相互转换。
J Mol Biol. 1968 Mar 14;32(2):159-68. doi: 10.1016/0022-2836(68)90001-6.

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