Aiking H, Sojka G
J Bacteriol. 1979 Aug;139(2):530-6. doi: 10.1128/jb.139.2.530-536.1979.
Rhodopseudomonas capsulata was grown under anaerobic, photosynthetic conditions in a continuous culture device. Under light limitation, at a constant dilution rate, it was shown that cell composition, including photopigment (bacteriochlorophyll and carotenoids) and ribonucleic acid content, was not affected by incident light intensity; however, steady state culture density varied directly and linearly with light intensity. On the other hand, photopigment and ribonucleic acid levels were affected by growth rate regardless of light intensity. Additional experiments indicated a high apparent Ks for growth of R. capsulata with respect to light. These results were interpreted to mean that near the maximum growth rate (D = 0.45 h-1) some internal metabolic process became the limiting factor for growth, rather than the imposed energy limitation. A mathematical expression for the relation between steady-state culture density and dilution rate was derived and was found to adequately describe the data. A strong correlation was found between continuous cultures limited either by light or by a chemical energy source.
荚膜红假单胞菌在连续培养装置中于厌氧光合条件下生长。在光照受限、稀释率恒定的情况下，研究表明细胞组成，包括光合色素（细菌叶绿素和类胡萝卜素）以及核糖核酸含量，不受入射光强度的影响；然而，稳态培养密度与光强度直接呈线性变化。另一方面，无论光强度如何，光合色素和核糖核酸水平受生长速率的影响。额外的实验表明荚膜红假单胞菌生长的表观Ks值较高。这些结果被解释为意味着在接近最大生长速率（D = 0.45 h-1）时，某些内部代谢过程成为生长的限制因素，而非施加的能量限制。推导了稳态培养密度与稀释率之间关系的数学表达式，发现该表达式能充分描述数据。在受光或化学能源限制的连续培养之间发现了很强的相关性。
