Montesinos E, Esteve I, Guerrero R
Appl Environ Microbiol. 1983 May;45(5):1651-8. doi: 10.1128/aem.45.5.1651-1658.1983.
Size frequency distributions of different phototrophic and heterotrophic microorganisms were determined by means of scanning and transmission electron microscopy and electronic particle sizing. Statistically significant differences existed among the three techniques used in this study. Cells processed for electron microscopy showed lower mean cellular volumes than those processed for electronic particle sizing, reflecting a shrinkage by factors ranging from 1.1 to 6.2 (mean, 2.3). Processing of cells for scanning electron microscopy caused higher shrinkage than processing for transmission electron microscopy. Shrinkage was dependent neither on the size nor on the cell wall type of the microorganism. When processed for scanning electron microscopy, phototrophic bacteria were strongly shrunken, whereas heterotrophic microorganisms were less affected. A direct relationship existed among phototrophic bacteria between percentage of shrinkage and specific pigment content. This was probably a consequence of the pigment extraction by organic solvents during the dehydration process, previous to the critical point drying, necessary to examine the specimens under the scanning electron microscope.
通过扫描电子显微镜、透射电子显微镜和电子颗粒大小测定法，确定了不同光合微生物和异养微生物的大小频率分布。本研究中使用的三种技术之间存在统计学上的显著差异。用于电子显微镜观察的细胞显示出比用于电子颗粒大小测定的细胞更低的平均细胞体积，这反映出细胞收缩了1.1至6.2倍（平均为2.3倍）。用于扫描电子显微镜观察的细胞处理过程比用于透射电子显微镜观察的细胞处理过程导致更高程度的收缩。收缩既不取决于微生物的大小，也不取决于其细胞壁类型。在进行扫描电子显微镜观察处理时，光合细菌强烈收缩，而异养微生物受影响较小。光合细菌的收缩百分比与特定色素含量之间存在直接关系。这可能是由于在临界点干燥之前的脱水过程中，有机溶剂提取了色素，而临界点干燥是在扫描电子显微镜下检查标本所必需的。
