通过高分辨率放射自显影观察两栖类卵母细胞核仁中的转录机制。

Mechanisms of transcription in nucleoli of amphibian oocytes as visualized by high-resolution autoradiography.

作者信息

Angelier N, Hemon D, Bouteille M

出版信息

J Cell Biol. 1979 Feb;80(2):277-90. doi: 10.1083/jcb.80.2.277.

DOI:10.1083/jcb.80.2.277

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2110331/

Abstract

In oocytes of Pleurodeles waltlii, the method of Miller and Beatty has been combined with a method of high-resolution autoradiography especially suitable for the study of isolated molecules. In vitro labeling of RNA by tritiated precursors was carried out with increasing incubation times (1, 4, 15, 24, 48, and 72 h). Silver grains were present over ribonucleoprotein fibrils in amounts sufficient for quantitative analysis of nucleolar DNA transcription. Statistical analysis of the data revealed that: (a) The units of any one nucleolus exhibited a large degree of heterogeneity in their number of grains. (b) There was a parallelism between the increasing grain number and the ribonucleoprotein-fibril lengthening as observed along the transcription unit.

摘要

在肋突螈的卵母细胞中，米勒和比蒂的方法已与一种特别适用于分离分子研究的高分辨率放射自显影方法相结合。用氚标记的前体对RNA进行体外标记，并延长孵育时间（1、4、15、24、48和72小时）。核糖核蛋白纤维上存在足够数量的银颗粒，可用于对核仁DNA转录进行定量分析。对数据的统计分析表明：（a）任何一个核仁的单位在其颗粒数量上表现出很大程度的异质性。（b）沿着转录单位观察到，颗粒数量的增加与核糖核蛋白纤维的延长之间存在平行关系。

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Evidence for a particular mode of transcription in globular loops of lampbrush chromosomes of the newt Pleurodeles waltlii.蝾螈（Pleurodeles waltlii）灯刷染色体球状环中特定转录模式的证据。

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本文引用的文献

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AUTORADIOGRAPHY WITH THE ELECTRON MICROSCOPE. A PROCEDURE FOR IMPROVING RESOLUTION, SENSITIVITY, AND CONTRAST.电子显微镜放射自显影术。一种提高分辨率、灵敏度和对比度的方法。

J Cell Biol. 1964 Aug;22(2):469-77. doi: 10.1083/jcb.22.2.469.

2

AMPHIBIAN CELL CULTURE: PERMANENT CELL LINE FROM THE BULLFROG (RANA CATESBEIANA).两栖动物细胞培养：源自牛蛙（北美牛蛙）的永久细胞系。

Science. 1964 Jun 26;144(3626):1578-80. doi: 10.1126/science.144.3626.1578.

3

Relative roles of the pituitary, follicle cells, and progesterone in the induction of oocyte maturation in Rana pipiens.垂体、卵泡细胞和孕酮在豹蛙卵母细胞成熟诱导中的相对作用。

J Exp Zool. 1967 Dec;166(3):365-75. doi: 10.1002/jez.1401660309.

4

Morphological studies of transcription.转录的形态学研究。

Acta Endocrinol Suppl (Copenh). 1972;168:155-77. doi: 10.1530/acta.0.071s155.

5

Morphology of ribosomal RNA cistrons in oocytes of the water beetle, Dytiscus marginalis L.水甲虫（Dytiscus marginalis L.）卵母细胞中核糖体RNA顺反子的形态学

Chromosoma. 1974;48(2):119-35. doi: 10.1007/BF00283959.

6

Regulation of transcription of genes of ribosomal rna during amphibian oogenesis. A biochemical and morphological study.两栖类卵子发生过程中核糖体RNA基因转录的调控。一项生化与形态学研究。

J Cell Biol. 1976 May;69(2):465-89. doi: 10.1083/jcb.69.2.465.

7

Electron microscopic autoradiography of isolated nucleolar transcription units.分离的核仁转录单位的电子显微镜放射自显影术。

Exp Cell Res. 1976 Jul;100(2):389-93. doi: 10.1016/0014-4827(76)90163-4.

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