Muller H K, Healy D L
Immunology. 1973 Jun;24(6):1099-112.
Active cutaneous anaphylaxis was studied in guinea-pigs sensitized by inhalation of egg albumen powder and producing high titre IgGγ1 and low titre IgE homocytotropic antibodies. On cutaneous antigen challenge an immediate increase in venular permeability occurred which declined rapidly over the next hour, normal permeability being restored by 2 hours. This was followed by a biphasic tissue eosinophilia with peaks at 3–6 hours and 14–16 hours. Tissue neutrophilia was also maximal at 14–16 hours with a preceding `shoulder' between 6 and 12 hours. Injection of antigen into non-sensitized animals produced only the early peak of leucocytosis. Fluorescent protein tracing demonstrated that little antigen persisted in lesions older than 7 hours. Neutrophil phagocytosis of antigen was maximal between 1 and 3 hours; eosinophil phagocytosis of antigen was not observed. IgGγ1 and IgGγ2 were localized to granulocytes during the 3–6 hour peak of leucocytosis, but not in older lesions. IgGγ was detected in eosinophils, but which granulocytes contained IgGγ2 antibodies remained uncertain. Binding of IgGγ1 antibodies to mast cells was not observed and C3 was not detected in any lesion. It is concluded that antigen induced the first peak of tissue leucocytosis; possible mediators of the second leucocytic peak include eosinophil chemotactic factor of anaphylaxis, mast cell products and leucoegresin.
在通过吸入蛋清粉致敏并产生高滴度IgGγ1和低滴度IgE亲细胞性抗体的豚鼠中研究了主动皮肤过敏反应。在皮肤抗原激发后，小静脉通透性立即增加，在接下来的一小时内迅速下降，2小时后恢复正常通透性。随后是双相组织嗜酸性粒细胞增多，在3 - 6小时和14 - 16小时达到峰值。组织嗜中性粒细胞增多在14 - 16小时也达到最大值，在6至12小时之间有一个先前的“肩部”。向未致敏动物注射抗原仅产生白细胞增多的早期峰值。荧光蛋白追踪显示，在超过7小时的病变中几乎没有抗原残留。抗原的中性粒细胞吞噬作用在1至3小时之间最大；未观察到抗原的嗜酸性粒细胞吞噬作用。在白细胞增多的3 - 6小时峰值期间，IgGγ1和IgGγ2定位于粒细胞，但在较老的病变中没有。在嗜酸性粒细胞中检测到IgGγ，但哪些粒细胞含有IgGγ2抗体仍不确定。未观察到IgGγ1抗体与肥大细胞的结合，并且在任何病变中均未检测到C3。结论是抗原诱导了组织白细胞增多的第一个峰值；第二个白细胞峰值的可能介质包括过敏反应嗜酸性粒细胞趋化因子、肥大细胞产物和白细胞脱落素。
