Kano M, Suzuki N, Ojima H
J Cell Physiol. 1979 Jun;99(3):327-31. doi: 10.1002/jcp.1040990307.
The effect of the presence of nerve extracts on the development of tetrodotoxin (TTX)-sensitive sodium channels in cultures of dissociated embryonic chick skeletal muscle cells was examined by measuring the maximum rate of rise of TTX-sensitive spike potential. The addition of the nerve extract prepared from brain or spinal cord of chick embryos to the culture medium caused an increase in the channel density. Extracts of non-neural tissues, i.e., lung, kidney, and muscle, were ineffective. Liver extract, however, produced an effect similar to the nerve extracts. These results suggest that the TTX-sensitive sodium channels in the muscle cell membrane are regulated by a diffusible chemical substance independently of innervation, and that this substance resides in neural tissues, and perhaps also in liver.
通过测量河豚毒素（TTX）敏感的锋电位上升的最大速率，研究了神经提取物对解离的胚胎鸡骨骼肌细胞培养物中TTX敏感钠通道发育的影响。将从鸡胚脑或脊髓制备的神经提取物添加到培养基中会导致通道密度增加。非神经组织（即肺、肾和肌肉）的提取物无效。然而，肝提取物产生了与神经提取物类似的效果。这些结果表明，肌细胞膜中的TTX敏感钠通道受一种可扩散的化学物质调节，与神经支配无关，并且这种物质存在于神经组织中，也可能存在于肝脏中。
