尿苷二磷酸6-脱氧半乳糖对大肠杆菌尿苷二磷酸半乳糖4-差向异构酶的竞争性抑制作用及底物活性
Competitive inhibition and substrate activity of uridine diphosphate 6-deoxygalactose for Escherichia coli uridine diphosphate galactose 4-epimerase.
作者信息
Spencer M, Blackburn P, Ferdinand W, Blackburn G M
出版信息
Biochem J. 1973 Feb;131(2):421-3. doi: 10.1042/bj1310421.
Abstract
UDP-6-deoxygalactose inhibits the UDP-galactose 4-epimerase (EC 5.1.3.2) from Escherichia coli in a competitive manner with respect to the substrate UDP-galactose, giving K(i) 1.3x10(-3)m. As a substrate for the enzyme, it is transformed into UDP-6-deoxyglucose, although the reaction stops before equilibrium is attained. Possible causes of this behaviour are discussed.
摘要
UDP-6-脱氧半乳糖以与底物UDP-半乳糖竞争的方式抑制来自大肠杆菌的UDP-半乳糖4-表异构酶（EC 5.1.3.2），抑制常数K(i)为1.3×10⁻³m。作为该酶的一种底物，它被转化为UDP-6-脱氧葡萄糖，尽管反应在达到平衡之前就停止了。文中讨论了这种行为可能的原因。
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引用本文的文献
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The concerted inactivation of Escherichia coli uridine diphosphate galactose 4-epimerase by sugar nucleotide together with a free sugar.糖核苷酸与游离糖协同使大肠杆菌尿苷二磷酸半乳糖4-表异构酶失活。
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本文引用的文献
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HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES.大肠杆菌突变体中半乳糖代谢的遗传性缺陷，I. 酶活性的测定
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Synthesis and proof of structure of tritiated glucose and its use in the study of the mechanism of UDPGal-C-4-epimerase.氚标记葡萄糖的合成、结构证明及其在UDPGal-C-4-表异构酶作用机制研究中的应用。
Biochem Biophys Res Commun. 1963 Jul 18;12:152-6. doi: 10.1016/0006-291x(63)90253-5.
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The determination of enzyme inhibitor constants.酶抑制剂常数的测定
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A vacuum system for the synthesis of nucleotide sugars on a micro scale.一种用于微量合成核苷酸糖的真空系统。
Anal Biochem. 1965 Dec;13(3):405-11. doi: 10.1016/0003-2697(65)90333-7.
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[The interaction of UDPG-4-epimerase with UDP-deoxyhexoses].[UDPG-4-表异构酶与UDP-脱氧己糖的相互作用]
Biokhimiia. 1969 May-Jun;34(3):518-23.
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Changes in the specific activities of the galactose enzymes in E. coli under different growth conditions.不同生长条件下大肠杆菌中半乳糖酶的比活性变化。
Mol Gen Genet. 1967;100(2):203-9. doi: 10.1007/BF00333606.
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