丙二酰辅酶A在类异戊二烯生物合成中的作用研究。
An investigation into the role of malonyl-coenzyme A in isoprenoid biosynthesis.
作者信息
Higgins M J, Kekwick R G
出版信息
Biochem J. 1973 May;134(1):295-310. doi: 10.1042/bj1340295.
Abstract
- [(14)C]Malonyl-CoA was incorporated into isoprenoids by cell-free yeast preparations, by preparations from pigeon and rat liver, and by Hevea brasiliensis latex. 2. In agreement with previous reports the incorporation of acetyl-CoA into isoprenoids was not inhibited by avidin and was not stimulated by HCO(3) (-). In a cell-free yeast preparation addition of HCO(3) (-) stimulated the formation of fatty acids from acetyl-CoA and decreased the incorporation into unsaponifiable lipids. 3. The labelling patterns of beta-hydroxy-beta-methylglutaryl-CoA formed from [2-(14)C]- and [1,3-(14)C]-malonyl-CoA in rat and pigeon liver preparations were those that would be expected if malonyl-CoA underwent decarboxylation to acetyl-CoA before incorporation. 4. The labelling pattern of ergosterol formed by cell-free yeast preparations from [2-(14)C]malonyl-CoA was also consistent with decarboxylation of malonyl-CoA before incorporation. 5. The incorporation of [2-(14)C]malonyl-CoA into mevalonate by rat liver preparations was related to the malonyl-CoA decarboxylase activity present in the preparation.
摘要
- [14C]丙二酰辅酶A可被无细胞酵母制剂、鸽肝和大鼠肝制剂以及巴西橡胶树胶乳掺入类异戊二烯中。2. 与先前的报道一致，抗生物素蛋白不抑制乙酰辅酶A掺入类异戊二烯，且碳酸氢根（HCO₃⁻）也不刺激其掺入。在无细胞酵母制剂中，添加碳酸氢根（HCO₃⁻）可刺激由乙酰辅酶A形成脂肪酸，并减少其掺入不皂化脂质中。3. 在大鼠和鸽肝制剂中，由[2-(14)C]-和[1,3-(14)C]-丙二酰辅酶A形成的β-羟基-β-甲基戊二酰辅酶A的标记模式，是如果丙二酰辅酶A在掺入前先脱羧形成乙酰辅酶A时所预期的模式。4. 无细胞酵母制剂由[2-(14)C]丙二酰辅酶A形成麦角固醇的标记模式也与丙二酰辅酶A在掺入前脱羧一致。5. 大鼠肝制剂将[2-(14)C]丙二酰辅酶A掺入甲羟戊酸的过程与制剂中存在的丙二酰辅酶A脱羧酶活性有关。
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An investigation into the role of malonyl-coenzyme A in isoprenoid biosynthesis.丙二酰辅酶A在类异戊二烯生物合成中的作用研究。
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本文引用的文献
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The biosynthesis of mevalonic acid from 1-C14-acetate by a rat liver enzyme system.大鼠肝脏酶系统由1-C14-乙酸盐生物合成甲羟戊酸。
J Biol Chem. 1959 Nov;234:2835-40.
2
Qualitative and quantitative determination of the fatty acids by gas-liquid chromatography.通过气液色谱法对脂肪酸进行定性和定量测定。
Methods Biochem Anal. 1960;8:1-59. doi: 10.1002/9780470110249.ch1.
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THE OCCURRENCE AND METABOLISM IN VITRO OF UNESTERIFIED FATTY ACID IN MOUSE BRAIN.小鼠脑中游离脂肪酸的体外发生与代谢
Biochim Biophys Acta. 1964 Aug 5;84:424-34. doi: 10.1016/0926-6542(64)90006-x.
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ENZYME-BOUND INTERMEDIATES IN THE BIOSYNTHESIS OF MEVALONIC AND PALMITIC AICDS.甲羟戊酸和棕榈酸生物合成过程中的酶结合中间体
J Biol Chem. 1964 May;239:1346-56.
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AVIDIN. 3. THE NATURE OF THE BIOTIN-BINDING SITE.抗生物素蛋白。3. 生物素结合位点的性质。
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AVIDIN. 2. PURIFICATION AND COMPOSITION.抗生物素蛋白。2. 纯化与组成。
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