Van Regenmortel M H, Hardie G
J Immunol Methods. 1979 May 10;27(1):43-54. doi: 10.1016/0022-1759(79)90237-0.
Binding studies with tobacco mosaic virus and specific IgG and Fab' fragments were done under conditions of 50% binding of antibody. When the log of the concentration of antigen sites required to bind 50% of the available antibody was plotted against the log of bound sites, a line was obtained which had a slope varying between 0 and 1. Instead of using the slope of this line as an index of avidity of the antiserum, it is suggested that the calculation K0 values over a range of reactant concentrations provides a better criterion for comparing the avidity of antiviral antibodies. The results of conventional binding tests were analyzed by means of Scatchard plots and were found to agree very closely with the data obtained at 50% binding of antibody.
在抗体50%结合的条件下，开展了烟草花叶病毒与特异性IgG及Fab’片段的结合研究。当将结合50%可用抗体所需抗原位点浓度的对数与结合位点的对数作图时，得到一条斜率在0到1之间变化的直线。建议不要将这条直线的斜率用作抗血清亲和力的指标，而是在一系列反应物浓度范围内计算K0值，这为比较抗病毒抗体的亲和力提供了更好的标准。通过Scatchard图对传统结合试验的结果进行分析，发现其与在抗体50%结合时获得的数据非常吻合。
