经γ射线照射的原生质体化大肠杆菌CR thy-细胞中细胞脱氧核糖核酸的降解
Degradation of cellular deoxyribonucleic acid after gamma irradiation of spheroplasted Escherichia coli CR thy- cells.
作者信息
Christensen R C, Keller K, Pollard E C
出版信息
J Bacteriol. 1973 Oct;116(1):491-3. doi: 10.1128/jb.116.1.491-493.1973.
Abstract
Gamma-ray-irradiated Escherichia coli CR thy(-) cells and spheroplasts, prelabeled with (14)C-thymine, were assayed for acid-insoluble activity as a function of incubation time after irradiation. Under similar irradiation and incubation conditions, degradation profiles of cells and spheroplasts were virtually identical. Similar results were found for cells and protoplasts irradiated in the presence of rifampin (20 mug/ml). These results suggest that postirradiation deoxyribonucleic acid degradation enzymes are probably not loosely localized in the periplasm, unlike endonuclease I.
摘要
用（14）C - 胸腺嘧啶预标记的经γ射线辐照的大肠杆菌CR thy（-）细胞和原生质球，在辐照后作为孵育时间的函数测定其酸不溶性活性。在相似的辐照和孵育条件下，细胞和原生质球的降解曲线几乎相同。在利福平（20微克/毫升）存在下辐照的细胞和原生质体也得到了类似结果。这些结果表明，与内切核酸酶I不同，辐照后脱氧核糖核酸降解酶可能不是松散地定位于周质中。
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Degradation of cellular deoxyribonucleic acid after gamma irradiation of spheroplasted Escherichia coli CR thy- cells.经γ射线照射的原生质体化大肠杆菌CR thy-细胞中细胞脱氧核糖核酸的降解
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Action of ionizing radiation on sensitive strains of Escherichia coli B.电离辐射对大肠杆菌B敏感菌株的作用
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