大肠杆菌核糖体蛋白与N-乙基马来酰亚胺反应的动力学
Kinetics of reaction of Escherichia coli ribosomal proteins toward N-ethylmaleimide.
作者信息
Rosenberg M, Berman D, Chang F N
出版信息
J Bacteriol. 1973 Oct;116(1):497-9. doi: 10.1128/jb.116.1.497-499.1973.
Abstract
The kinetics of reaction of the exposed ribosomal proteins from Escherichia coli toward N-ethylmaleimide (NEM) have been studied. While most of the proteins from the 30S subunit react rapidly with NEM and plateau levels can be reached, most of the proteins from the 50S subunit show biphasic uptake kinetics. Furthermore, differences have been observed in the reactivity of proteins toward NEM depending on whether the protein is in the free subunit or in the 70S ribosome.
摘要
对来自大肠杆菌的暴露核糖体蛋白与N-乙基马来酰亚胺（NEM）的反应动力学进行了研究。虽然30S亚基的大多数蛋白与NEM反应迅速且能达到平稳水平，但50S亚基的大多数蛋白呈现双相摄取动力学。此外，根据蛋白是处于游离亚基还是70S核糖体中，观察到了蛋白对NEM反应性的差异。
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