胸腺嘧啶饥饿对大肠杆菌CR34（λ ind-）中附加体和染色体脱氧核糖核酸完整性的影响
Influence of thymine starvation on the integrity of episomal and chromosomal deoxyribonucleic acids in Escherichia coli CR34 (lambda ind-).
作者信息
Shuster R C
出版信息
J Bacteriol. 1973 Nov;116(2):1067-70. doi: 10.1128/jb.116.2.1067-1070.1973.
Abstract
The effect of thymine deprivation on the integrity of phage lambda, sex factor, and chromosomal deoxyribonucleic acid (DNA) in Escherichia coli CR34 (lambda ind(-)) was examined by sedimenting cell lysates through alkaline sucrose gradients. Both sex factor and chromosomal DNAs showed evidence of being degraded during the starvation period. In contrast, no loss of closed circular lambda DNA was observed.
摘要
通过在碱性蔗糖梯度中沉降细胞裂解物，研究了胸腺嘧啶剥夺对大肠杆菌CR34（λ ind(-)）中λ噬菌体、性因子和染色体脱氧核糖核酸（DNA）完整性的影响。在饥饿期间，性因子和染色体DNA均显示出被降解的迹象。相比之下，未观察到闭环λ DNA的损失。
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