噬菌体T5裸露的、部分注入（FST后）的DNA对宿主细胞的穿透
Penetration into host cells of naked, partially injected (post-FST) DNA of bacteriophage T5.
作者信息
Labedan B, Legault-Demare J
出版信息
J Virol. 1973 Aug;12(2):226-9. doi: 10.1128/JVI.12.2.226-229.1973.
Abstract
The experiments presented here suggest that the naked post-FST DNA, attached to the bacteria after a centrifugation at 6,000 x g of Escherichia coli-T5 complexes arrested at the FST stage, can be injected into the host cell if protein synthesis is allowed. This results from the observation that the production of infectious centers by this naked DNA is sensitive to DNase or moderate shearing treatments. The process of injection seems to be the same as that for DNA normally rolled in an adsorbed capsid.
摘要
此处展示的实验表明，在6000×g离心后附着于处于FST阶段停滞的大肠杆菌-T5复合物上的裸FST后DNA，如果允许蛋白质合成，可被注入宿主细胞。这是基于以下观察结果：这种裸DNA产生感染中心的过程对DNase或适度剪切处理敏感。注入过程似乎与正常包裹在吸附衣壳中的DNA相同。
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