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N-甲基异吲哚酮-β-硫代半卡巴腙-铜配合物与蛋白质和核酸的结合

Binding of N-methyl isatin beta-thiosemicarbazone-copper complexes to proteins and nucleic acids.

作者信息

Rohde W, Shafer R, Idriss J, Levinson W

出版信息

J Inorg Biochem. 1979 Jun;10(3):183-94. doi: 10.1016/s0162-0134(00)80278-0.

DOI:10.1016/s0162-0134(00)80278-0
PMID:458443
Abstract

N-Methyl isatin beta-thiosemicarbazone-copper complexes interact with nucleic acids and proteins as shown by ultraviolet (UV) and visible spectroscopy and Sephadex exclusion chromatography. The Cu++ ions are most effective; Co++ ions have less albeit significant activity. Chelating agents, such as Tris and histidine, high NaCl concentration, and dimethyl sulfoxide reduce the binding of the drug-metal complex. The binding constant of the drug-copper complex to calf-thymus DNA was calculated to range between 6.9 x 10(4) and 2.7 x 10(5) M-1.

摘要

N-甲基异吲哚啉酮β-硫代半卡巴腙-铜配合物与核酸和蛋白质相互作用，这通过紫外（UV）和可见光谱以及葡聚糖凝胶排阻色谱得以证明。Cu++离子最为有效；Co++离子的活性虽较低但也很显著。螯合剂，如Tris和组氨酸、高浓度的NaCl以及二甲基亚砜会降低药物-金属配合物的结合。药物-铜配合物与小牛胸腺DNA的结合常数经计算在6.9×10(4)至2.7×10(5) M-1之间。

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