缺乏葡萄糖的T偶数噬菌体DNA的模板特性
Template properties of glucose-deficient T-even bacteriophage DNA.
作者信息
Cox G S, Conway T W
出版信息
J Virol. 1973 Dec;12(6):1279-87. doi: 10.1128/JVI.12.6.1279-1287.1973.
Abstract
The vegetative DNA isolated from T4-infected Escherichia coli W4597 (UDPG PPase(-)) was about two to six times more active in stimulating protein synthesis in cell-free extracts than that isolated from T4-infected E. coli B06. This suggested that nonglucosylated vegetative DNA may be a better template than the glucosylated form. This view was supported by experiments measuring RNA synthesis on mature T-even DNAs with a range of glucose contents. The extent of (14)C-GTP polymerization was inversely proportional to the glucose content of the DNA. Differences were also observed in both the kind and quantity of polypeptides produced in response to these DNAs.
摘要
从感染T4噬菌体的大肠杆菌W4597（UDPG焦磷酸酶阴性）中分离出的营养型DNA，在刺激无细胞提取物中的蛋白质合成方面，其活性比从感染T4噬菌体的大肠杆菌B06中分离出的营养型DNA高约两到六倍。这表明未糖基化的营养型DNA可能比糖基化形式的DNA是更好的模板。通过测量具有一系列葡萄糖含量的成熟T偶数DNA上的RNA合成的实验支持了这一观点。（14）C-GTP聚合的程度与DNA的葡萄糖含量成反比。在响应这些DNA产生的多肽的种类和数量上也观察到了差异。
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