Shaw M T, Nordquist R E
Cancer. 1975 Jan;35(1):208-14. doi: 10.1002/1097-0142(197501)35:1<208::aid-cncr2820350126>3.0.co;2-c.
In a series of 130 cases of acute leukemia studied by cytochemical staining techniques, 10 cases cytochemically diagnosed as "pure" monocytic leukemia were seen. Cytochemical staining of bone marrow aspirates from these patients revealed all leukemic cells to be Sudan black negative. No positive reactions were observed for peroxidase or naphthol AS-D chloroacetate esterase. All cases demonstrated strong alpha-naphthyl acetate esterase positivity; and fluoride-inhibited naphthol AS-D acetate esterase positivity was observed in 8 of 9 cases tested. The P.A.S. reaction showed diffuse fine to coarse granules. Oil red O stain was positive in 8 of 9 cases, and the beta-glucuronidase activity was strong in 5 of 9 cases. Light microscopy revealed cells with monocytic or histiocytic morphology. Electron microscopic studies in 2 cases demonstrated features consistent with leukemic monocytic or histiocytic morphology; none was suggestive of granulocytic or lymphocytic leukemia. Five of 6 patients treated with drug regimens including prednisone and vincristine entered a complete remission; the other obtained a partial remission. Two patients achieved complete remission after treatment with Adriamycin, 1 following a relapse. Three patients who received cytosine arabinoside as their only therapy died soon after treatment was commenced. It is suggested that the cytochemical similarity but morphological differences in those patients may be objectively used to group them as cases of histiomonocytic leukemia.
在用细胞化学染色技术研究的130例急性白血病病例系列中，发现10例经细胞化学诊断为“纯”单核细胞白血病。对这些患者骨髓穿刺液进行细胞化学染色显示，所有白血病细胞苏丹黑染色均为阴性。过氧化物酶或萘酚AS - D氯乙酸酯酶未观察到阳性反应。所有病例均显示α - 萘乙酸酯酶强阳性；在9例检测病例中的8例观察到氟化钠抑制的萘酚AS - D乙酸酯酶阳性。P.A.S.反应显示弥漫性细颗粒至粗颗粒。油红O染色在9例中的8例呈阳性，β - 葡萄糖醛酸酶活性在9例中的5例较强。光镜检查显示细胞具有单核细胞或组织细胞形态。2例的电镜研究显示其特征与白血病单核细胞或组织细胞形态一致；均未提示粒细胞或淋巴细胞白血病。6例接受包括泼尼松和长春新碱的药物方案治疗的患者中有5例完全缓解；另1例部分缓解。2例患者在接受阿霉素治疗后完全缓解，1例在复发后缓解。3例仅接受阿糖胞苷治疗的患者在治疗开始后不久死亡。提示这些患者在细胞化学上具有相似性但形态上存在差异，可客观地将其归为组织细胞单核细胞白血病病例。
