体外噬菌体蛋白特异性切割所需的T4诱导活性。
T4-induced activity required for specific cleavage of a bacteriophage protein in vitro.
作者信息
Goldstein J, Champe S P
出版信息
J Virol. 1974 Feb;13(2):419-27. doi: 10.1128/JVI.13.2.419-427.1974.
Abstract
We have examined the acid-soluble products formed during incubation of labeled substrate protein from T4-infected cells with unlabeled phage-infected cell extracts. If the substrate protein is prepared from cells infected with a T4 mutant blocked in cleavage of phage head precursor proteins, the products formed in vitro include a peptide indistinguishable by several criteria from one of the T4 internal peptides. Denatured as well as undenatured protein can serve as the substrate for the formation of this peptide. As expected, this peptide is not formed if protein from either uninfected cells or cells infected with wild-type T4 is used as substrate. The formation of this peptide in vitro is dependent on a factor present in extracts of phage-infected cells but absent from extracts of uninfected cells.
摘要
我们检测了用未标记的噬菌体感染细胞提取物孵育来自T4感染细胞的标记底物蛋白时形成的酸溶性产物。如果底物蛋白是从感染了在噬菌体头部前体蛋白切割中受阻的T4突变体的细胞中制备的，那么体外形成的产物包括一种在几个标准上与T4内部肽之一无法区分的肽。变性蛋白和未变性蛋白都可以作为形成这种肽的底物。正如预期的那样，如果使用来自未感染细胞或感染野生型T4的细胞的蛋白作为底物，则不会形成这种肽。这种肽在体外的形成依赖于噬菌体感染细胞提取物中存在但未感染细胞提取物中不存在的一种因子。
相似文献
1
T4-induced activity required for specific cleavage of a bacteriophage protein in vitro.体外噬菌体蛋白特异性切割所需的T4诱导活性。
J Virol. 1974 Feb;13(2):419-27. doi: 10.1128/JVI.13.2.419-427.1974.
2
Identification of gene products required for in vitro formation of the internal peptides of bacteriophage T4.噬菌体T4内部肽段体外形成所需基因产物的鉴定。
J Virol. 1976 Jun;18(3):894-903. doi: 10.1128/JVI.18.3.894-903.1976.
3
Cleavage of T4-induced proteins during phage morphogenesis: characterization of peptides.噬菌体形态发生过程中T4诱导蛋白的裂解：肽段的表征
J Gen Virol. 1976 May;31(2):163-72. doi: 10.1099/0022-1317-31-2-163.
4
Isolation by affinity chromatography of a precursor head protein (P23) of bacteriophage T4.
J Mol Biol. 1974 Aug 5;87(2):317-27. doi: 10.1016/0022-2836(74)90152-1.
5
Purification of a proteolytic enzyme from T4-infected Escherichia coli cells.从T4噬菌体感染的大肠杆菌细胞中纯化一种蛋白水解酶。
Virology. 1976 Jun;71(2):602-4. doi: 10.1016/0042-6822(76)90386-x.
6
Precursors of the T4 internal peptides.T4内部肽段的前体。
J Virol. 1977 May;22(2):412-9. doi: 10.1128/JVI.22.2.412-419.1977.
7
Bacteriophage M 13 gene 2 protein: increasing its yield in infected cells, and identification and localization.噬菌体M13基因2蛋白：提高其在感染细胞中的产量以及鉴定和定位
Virology. 1974 Oct;61(2):334-42. doi: 10.1016/0042-6822(74)90271-2.
8
Membrane protein synthesis after infection of Escherichia coli B with phage T4: the rIIB protein.用噬菌体T4感染大肠杆菌B后膜蛋白的合成：rIIB蛋白
Virology. 1972 Nov;50(2):520-7. doi: 10.1016/0042-6822(72)90403-5.
9
The selective inhibition of protein initiation by T4 phage-induced factors.T4噬菌体诱导因子对蛋白质起始的选择性抑制作用。
Proc Natl Acad Sci U S A. 1970 Oct;67(2):696-701. doi: 10.1073/pnas.67.2.696.
10
Particle containing uncleaved major head protein (P23) which is maturable into the head of phage T4.含有未切割的主要头部蛋白（P23）的颗粒，该蛋白可成熟为噬菌体T4的头部。
Nature. 1974 Jun 28;249(460):825-7. doi: 10.1038/249825a0.
引用本文的文献
1
Bacteriophage T4 genome.噬菌体T4基因组。
Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents. doi: 10.1128/MMBR.67.1.86-156.2003.
2
Separate functional domains of the herpes simplex virus type 1 protease: evidence for cleavage inside capsids.单纯疱疹病毒1型蛋白酶的独立功能结构域：衣壳内切割的证据
J Virol. 1996 Jul;70(7):4317-28. doi: 10.1128/JVI.70.7.4317-4328.1996.
3
Identification of gene products required for in vitro formation of the internal peptides of bacteriophage T4.噬菌体T4内部肽段体外形成所需基因产物的鉴定。
J Virol. 1976 Jun;18(3):894-903. doi: 10.1128/JVI.18.3.894-903.1976.
4
Structural aberrations in T-even bacteriophage. VII. In vitro analysis of the canavanine-mediated inhibition of proteolytic cleavage.T-偶数噬菌体中的结构畸变。VII. 刀豆氨酸介导的蛋白水解切割抑制作用的体外分析。
J Virol. 1975 Nov;16(5):1273-81. doi: 10.1128/JVI.16.5.1273-1281.1975.
5
Protein cleavage during virus assembly: a novel specificity of assembly dependent cleavage in bacteriophage T4.病毒组装过程中的蛋白质切割：噬菌体T4中依赖组装的切割的新特异性。
Proc Natl Acad Sci U S A. 1976 Nov;73(11):4205-9. doi: 10.1073/pnas.73.11.4205.
6
Characterization of DNA condensates induced by poly(ethylene oxide) and polylysine.聚环氧乙烷和聚赖氨酸诱导的DNA凝聚物的表征
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4288-92. doi: 10.1073/pnas.72.11.4288.
7
Precursors of the T4 internal peptides.T4内部肽段的前体。
J Virol. 1977 May;22(2):412-9. doi: 10.1128/JVI.22.2.412-419.1977.
8
Head length control in T4 bacteriophage morphogenesis: effect of canavanine on assembly.T4噬菌体形态发生过程中的头部长度控制：刀豆氨酸对组装的影响。
Bacteriol Rev. 1976 Jun;40(2):314-59. doi: 10.1128/br.40.2.314-359.1976.
9
Head morphogenesis of complex double-stranded deoxyribonucleic acid bacteriophages.复杂双链脱氧核糖核酸噬菌体的头部形态发生
Microbiol Rev. 1978 Sep;42(3):529-76. doi: 10.1128/mr.42.3.529-576.1978.
本文引用的文献
1
On a modification of the gene product P23 according to its use as subunit of either normal capsids of phage T4 or of polyheads.基于基因产物P23作为噬菌体T4正常衣壳亚基或多角体亚基的用途对其进行的修饰。
FEBS Lett. 1970 Jun 1;8(3):140-144. doi: 10.1016/0014-5793(70)80247-2.
2
Structural proteins of bacteriophage T4.噬菌体T4的结构蛋白
J Mol Biol. 1970 Nov 14;53(3):461-74. doi: 10.1016/0022-2836(70)90077-x.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Genetic determinant of an internal peptide of bacteriophage T4.噬菌体T4内部肽段的遗传决定因素。
J Mol Biol. 1969 Dec 28;46(3):377-92. doi: 10.1016/0022-2836(69)90183-1.
5
Components in T4-infected cells associated with phage assembly.T4感染细胞中与噬菌体组装相关的成分。
Virology. 1966 Nov;30(3):471-81. doi: 10.1016/0042-6822(66)90123-1.
6
Analysis of T4 phage proteins. I. Conversion of precursor proteins into lower molecular weight peptides during normal capsid formation.T4噬菌体蛋白质分析。I. 正常衣壳形成过程中前体蛋白转化为低分子量肽段。
Proc Natl Acad Sci U S A. 1970 Aug;66(4):1275-81. doi: 10.1073/pnas.66.4.1275.
7
Protein synthesis by Escherichia coli infected with bacteriophage T4D.被噬菌体T4D感染的大肠杆菌的蛋白质合成
Virology. 1968 Apr;34(4):709-27. doi: 10.1016/0042-6822(68)90092-5.
8
Assembly core of bacteriophage T4: an intermediate in head formation.噬菌体T4的装配核心：头部形成过程中的一个中间体。
Nat New Biol. 1973 Mar 21;242(116):70-5. doi: 10.1038/newbio242070a0.
9
Cleavage of viral precursor proteins in vivo and in vitro.病毒前体蛋白在体内和体外的切割
J Virol. 1972 Oct;10(4):751-9. doi: 10.1128/JVI.10.4.751-759.1972.
Zotero 插件