Beaudoin J, Pratt D
J Virol. 1974 Feb;13(2):466-9. doi: 10.1128/JVI.13.2.466-469.1974.
Antiserum inactivation experiments were carried out on electrophoretically purified diploid virions from a cross between two complementing amber mutants of phage M13. The total (homozygous plus heterozygous) diploid population, assayed on a permissive host where only one genome is needed for plaque formation, was inactivated at the same rate as haploids. Heterozygous diploids, assayed on a nonpermissive host, where both genomes are needed for plaque formation, were twice as sensitive as haploids and the total diploid population. These results have led us to propose a model for serum inactivation of the F-specific filamentous phages. According to this model, phage-neutralizing antibodies attach anywhere along the length of the phage and allow the phage to penetrate only up to the first bound antibody molecule.
对来自噬菌体M13的两个互补琥珀突变体杂交产生的经电泳纯化的二倍体病毒粒子进行了抗血清灭活实验。在允许性宿主上进行测定（在该宿主上形成噬菌斑仅需一个基因组），总的（纯合加杂合）二倍体群体的灭活速率与单倍体相同。在非允许性宿主上进行测定（在该宿主上形成噬菌斑需要两个基因组），杂合二倍体的敏感性是单倍体和总的二倍体群体的两倍。这些结果使我们提出了一个F特异性丝状噬菌体血清灭活的模型。根据该模型，噬菌体中和抗体附着在噬菌体长度的任何位置，并使噬菌体仅能穿透到第一个结合的抗体分子处。
