多胺刺激下氨酰基转移核糖核酸形成的表征
Characterization of aminoacyl transfer ribonucleic acid formation stimulated by polyamines.
作者信息
Takeda Y, Matsuzaki K, Igarashi K
出版信息
J Bacteriol. 1972 Jul;111(1):1-6. doi: 10.1128/jb.111.1.1-6.1972.
Abstract
From a comparative study of aminoacyl transfer ribonucleic acid (tRNA) formation stimulated by polyamines or by Mg(2+), it is shown that both reactions have the same requirements for adenosine triphosphate, tRNA, and aminoacyl-tRNA synthetase. Reaction kinetics are similar in both reactions. The same tRNA is aminoacylated in the presence of either spermine of Mg(2+). Aminoacyl-tRNA formed in the presence of polyamines can be a donor of amino acids in polypeptide synthesis.
摘要
通过对由多胺或镁离子（Mg²⁺）刺激形成氨酰转移核糖核酸（tRNA）的比较研究表明，这两种反应对三磷酸腺苷、tRNA和氨酰-tRNA合成酶具有相同的需求。两种反应的动力学相似。在精胺或镁离子（Mg²⁺）存在的情况下，同一种tRNA会被氨酰化。在多胺存在下形成的氨酰-tRNA可以作为多肽合成中氨基酸的供体。
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