F因子脱氧核糖核酸部分变性的电子显微镜分析
Electron microscope analysis of partial denaturation of F factor deoxyribonucleic acid.
作者信息
Hsu M T
出版信息
J Bacteriol. 1974 May;118(2):425-33. doi: 10.1128/jb.118.2.425-433.1974.
Abstract
Partial denaturation pattern of sex factor deoxyribonucleic acid of Escherichia coli was studied by electron microscopy. Clustering of the adenine-plusthymine-rich regions in one part of the molecule was revealed. The positions of these regions were located on the physical map of F by analyzing the partial denaturation pattern of heteroduplexes between F and F-prime factors with various parts of F sequences deleted.
摘要
通过电子显微镜研究了大肠杆菌性因子脱氧核糖核酸的部分变性模式。揭示了分子中富含腺嘌呤加胸腺嘧啶区域在一处的聚集现象。通过分析F与F - prime因子之间的异源双链体的部分变性模式（其中F的不同部分序列被删除），确定了这些区域在F的物理图谱上的位置。
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