Holt R J, Frankcombe C H, Newman R L
Arch Dis Child. 1974 Apr;49(4):318-21. doi: 10.1136/adc.49.4.318.
The need for frequent blood cultures from small babies for the early detection of bacteraemia associated with internal prosthesis colonization led to the development of a method for capillary blood collection. Fingers, toes, or heels were sterilized as well as possible, the surface was coated with sterile petroleum jelly, and sufficient blood was collected aseptically for aerobic broth culture and agar pour plates. From 242 patients, mostly babies with hydrocephalus and ventriculoatrial shunts, 740 cultures were collected; 135 (19%) cultures were positive and 32 (4·3%) were judged to be contaminated. A comparison between the results of capillary and venous blood cultures is made.
为了早期检测与体内假体定植相关的菌血症,需要从小婴儿身上频繁采集血培养样本,这促使了一种毛细血管采血方法的发展。尽可能对手指、脚趾或足跟进行消毒,在其表面涂抹无菌凡士林,然后无菌采集足够的血液用于需氧肉汤培养和琼脂倾注平板培养。从242名患者(大多数是患有脑积水和脑室心房分流术的婴儿)身上采集了740份培养样本;其中135份(19%)培养样本呈阳性,32份(4.3%)被判定为污染样本。本文对毛细血管血培养和静脉血培养的结果进行了比较。