新鲜鸡肉中粪大肠菌群的快速检测与计数方法
Rapid method for detection and enumeration of fecal coliforms in fresh chicken.
作者信息
Francis D W, Peeler J T, Twedt R M
出版信息
Appl Microbiol. 1974 Jun;27(6):1127-30. doi: 10.1128/am.27.6.1127-1130.1974.
A rapid method for enumerating fecal coliforms in foods was developed employing an agar pour-plate medium. After 7 h of incubation at 41.5 +/- 0.05 C, this medium effectively allows the growth of fecal coliforms only. This rapid method was compared with the Association of Official Analytical Chemists multiple-tube dilution method for Escherichia coli, by using 21 samples of fresh, cut-up chicken and a surface rinsing procedure for sample preparation. Verification of picked colonies was carried out in EC broth using parallel incubation temperatures of 45.5 and 44.5 +/- 0.05 C. Verifications for these temperatures averaged 79 and 98%, respectively. All positively verified isolates were E. coli types I and II, as were the negatives. Geometric means for the verified 7-h plate count were within 12% of the standard means for both EC broth incubation temperatures.
开发了一种使用琼脂倾注平板培养基来计数食品中粪大肠菌群的快速方法。在41.5±0.05℃下培养7小时后，这种培养基仅能有效地使粪大肠菌群生长。通过使用21份新鲜切碎鸡肉样品并采用表面冲洗程序进行样品制备，将这种快速方法与美国官方分析化学家协会的大肠杆菌多管稀释法进行了比较。使用45.5℃以及44.5±0.05℃的平行培养温度在EC肉汤中对挑选出的菌落进行验证。这些温度下的验证平均分别为79%和98%。所有经阳性验证的分离株均为I型和II型大肠杆菌，阴性分离株也是如此。对于两种EC肉汤培养温度，经验证的7小时平板计数的几何平均值均在标准平均值的12%以内。
Zotero 插件