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Millipore filtration assay for AUG-directed met-tRNAf binding to 40 S and 80 S ribosomes.

作者信息

Chatterjee B, Dasgupta A, Majumdar A, Palmieri S, Gupta N K

出版信息

Methods Enzymol. 1979;60:256-65. doi: 10.1016/s0076-6879(79)60023-x.

DOI:10.1016/s0076-6879(79)60023-x
PMID:459901
Abstract
摘要

相似文献

1
Millipore filtration assay for AUG-directed met-tRNAf binding to 40 S and 80 S ribosomes.
Methods Enzymol. 1979;60:256-65. doi: 10.1016/s0076-6879(79)60023-x.
2
Protein synthesis in rabbit reticulocytes. A study of Met-tRNA f Met binding factor(s) and Met-tRNA f Met binding to ribosomes and AUG codon.兔网织红细胞中的蛋白质合成。对甲硫氨酰 - tRNAfMet 结合因子及甲硫氨酰 - tRNAfMet 与核糖体和 AUG 密码子结合的研究。
J Biol Chem. 1975 Feb 10;250(3):853-62.
3
The binding of Met-tRNAf to isolated 40-S ribosomal subunits and the formation of Met-tRNAf - 80-S-ribosome initiation complexes.甲硫氨酰 - 起始tRNA与分离的40 - S核糖体亚基的结合以及甲硫氨酰 - 起始tRNA - 80 - S核糖体起始复合物的形成。
Eur J Biochem. 1976 Feb 16;62(2):243-55. doi: 10.1111/j.1432-1033.1976.tb10154.x.
4
Assays for investigating the regulation of Met-tRNAf binding activity.用于研究甲硫氨酸起始tRNA结合活性调控的分析方法。
Methods Enzymol. 1979;60:281-90. doi: 10.1016/s0076-6879(79)60026-5.
5
Reduced formation of initiation complexes between Met-tRNAf and 40-S ribosomal subunits in rabbit reticulocyte lysates incubated at elevated temperatures. Activity of the Met-tRNAf binding factor.
Eur J Biochem. 1978 Mar 15;84(2):601-10. doi: 10.1111/j.1432-1033.1978.tb12203.x.
6
Assay of the binding of the ternary complex Met-tRNAf.eIF-2.GTP to the 40 S ribosomal subunit by sucrose gradient and CsCl gradient analysis.通过蔗糖梯度和氯化铯梯度分析来检测三元复合物甲硫氨酰 - 起始tRNA.f.eIF - 2.GTP与40S核糖体亚基的结合情况。
Methods Enzymol. 1979;60:275-80. doi: 10.1016/s0076-6879(79)60025-3.
7
Protein synthesis in rabbit reticulocytes. Characteristics of mRNA (AUG codon)-dependent binding of Met-tRNAfMet to 40 S and 80 S ribosomes.兔网织红细胞中的蛋白质合成。甲硫氨酰 - 起始tRNA（Met-tRNAfMet）与40S和80S核糖体的mRNA（AUG密码子）依赖性结合的特征。
J Biol Chem. 1976 Oct 25;251(20):6379-87.
8
Purification of protein synthesis initiation factors from rabbit reticulocytes.
Methods Enzymol. 1979;60:101-8. doi: 10.1016/s0076-6879(79)60010-1.
9
Analysis of initiation factor function in highly fractionated and unfractionated reticulocyte lysate systems.
Methods Enzymol. 1979;60:61-87. doi: 10.1016/s0076-6879(79)60008-3.
10
Purification and characterization of seven initiation factors for mammalian protein synthesis.
Methods Enzymol. 1979;60:136-65. doi: 10.1016/s0076-6879(79)60013-7.

引用本文的文献

1
Protein synthesis in rabbit reticulocytes: characteristics of a postribosomal supernatant factor that reverses inhibition of protein synthesis in heme-deficient lysates and inhibition of ternary complex (Met-tRNAfMet.eIF-2.GTP) formation by heme-regulated inhibitor.兔网织红细胞中的蛋白质合成：一种核糖体后上清因子的特性，该因子可逆转血红素缺乏裂解物中蛋白质合成的抑制以及血红素调节抑制剂对三元复合物（Met-tRNAfMet.eIF-2.GTP）形成的抑制。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5490-4. doi: 10.1073/pnas.76.11.5490.
2
Protein synthesis in rabbit reticulocytes: mechanism of protein synthesis inhibition by heme-regulated inhibitor.兔网织红细胞中的蛋白质合成：血红素调节抑制剂对蛋白质合成的抑制机制。
Proc Natl Acad Sci U S A. 1979 Oct;76(10):5076-9. doi: 10.1073/pnas.76.10.5076.