通过蔗糖梯度和氯化铯梯度分析来检测三元复合物甲硫氨酰 - 起始tRNA.f.eIF - 2.GTP与40S核糖体亚基的结合情况。 - Suppr | 超能文献

Assay of the binding of the ternary complex Met-tRNAf.eIF-2.GTP to the 40 S ribosomal subunit by sucrose gradient and CsCl gradient analysis.

作者信息

Henshaw E C

出版信息

Methods Enzymol. 1979;60:275-80. doi: 10.1016/s0076-6879(79)60025-3.

DOI:10.1016/s0076-6879(79)60025-3

Abstract

摘要

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Assay of the binding of the ternary complex Met-tRNAf.eIF-2.GTP to the 40 S ribosomal subunit by sucrose gradient and CsCl gradient analysis.通过蔗糖梯度和氯化铯梯度分析来检测三元复合物甲硫氨酰 - 起始tRNA.f.eIF - 2.GTP与40S核糖体亚基的结合情况。

Methods Enzymol. 1979;60:275-80. doi: 10.1016/s0076-6879(79)60025-3.

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Preparation and characterization of eukaryotic initiation factor EIF-3. Formation of binary (EIF-3-Met-tRNAf) and ternary (EIF-3-Met-tRNAf-GTP) complexes.真核起始因子EIF-3的制备与表征。二元复合物（EIF-3-甲硫氨酰-tRNAf）和三元复合物（EIF-3-甲硫氨酰-tRNAf-GTP）的形成。

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The activity of eukaryotic initiation factor eIF-2 in ternary complex formation with GTP and Met-tRNA.真核生物起始因子eIF-2在与GTP和甲硫氨酰-tRNA形成三元复合物中的活性。

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The binding of Met-tRNAf to isolated 40-S ribosomal subunits and the formation of Met-tRNAf - 80-S-ribosome initiation complexes.甲硫氨酰 - 起始tRNA与分离的40 - S核糖体亚基的结合以及甲硫氨酰 - 起始tRNA - 80 - S核糖体起始复合物的形成。

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Protein synthesis in rabbit reticulocytes XX: a supernatant factor (TDI) inhibits ternary complex (Met-tRNAf-EIF-1-GTP) dissociation and Met-tRNAf binding to 40S ribosomes.兔网织红细胞中的蛋白质合成XX：一种上清液因子（TDI）抑制三元复合物（甲硫氨酸- tRNAf - EIF - 1 - GTP）的解离以及甲硫氨酸- tRNAf与40S核糖体的结合。

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Purification and properties of rabbit reticulocyte protein synthesis initiation factors EIF-1, EIF-2, and EIF-3.兔网织红细胞蛋白质合成起始因子EIF-1、EIF-2和EIF-3的纯化及特性

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Protein synthesis in rabbit reticulocytes. A study of Met-tRNA f Met binding factor(s) and Met-tRNA f Met binding to ribosomes and AUG codon.兔网织红细胞中的蛋白质合成。对甲硫氨酰 - tRNAfMet 结合因子及甲硫氨酰 - tRNAfMet 与核糖体和 AUG 密码子结合的研究。

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Regulation of protein synthesis in rabbit reticulocyte lysates: additional initiation factor required for formation of ternary complex (eIF-2.GTP.Met-tRNAf) and demonstration of inhibitory effect of heme-regulated protein kinase.兔网织红细胞裂解物中蛋白质合成的调控：三元复合物（eIF-2·GTP·Met-tRNAf）形成所需的额外起始因子以及血红素调节蛋白激酶抑制作用的证明。

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Initiation of the polypeptide chain by reticulocyte cell-free systems. Survey of different inhibitors of translation.网织红细胞无细胞系统引发多肽链。翻译的不同抑制剂研究。

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