Limitation of resistance as a parameter by which to characterize epithelia that actively transport ions.

It is theoretically inappropriate to characterize an actively transporting epithelim by "resistance" alone which is correctly applied only to passive circuit elements. Rather, such epithelia (if they actively transport sodium) required, as a minimum, characterization by an active circuit element parameter such as voltage (E Na), current (I Na) or power (WNa) in some configuration with resistances. Recent experimental studies of epithelia which actively transport sodium have omitted consideration of the active circuit element and attributed all measured changes observed to "resistance" changes in the epithelium as the transepithelial sodium gradient is altered. It is suggested that the observed changes in voltage/current ratio could be consequences of changes in the electrical behavior of the active circuit element of such epithelia. It may be biologically impossible to suppress all electromotive forces in epithelia to measure the truly passive characteristics of the epithelim without destroying tissue viability. The actual methods used to date for measurement of "resistance" in epithelia consist of perturbing signals which might alter the electrical behavior of an active element such as an "ion pump"; the observed changes in voltage to current ratio observed in such experiments can be better explained by a change in the active ion pump rather than by changes in passive epithelial "resistance".