Konrad M W
J Bacteriol. 1974 Jul;119(1):228-41. doi: 10.1128/jb.119.1.228-241.1974.
The kinetics of radioactive phosphate incorporation into the adenosine and guanosine nucleoside triphosphate termini of bacterial ribonucleic acid (RNA) was studied. Knowledge obtained in a previous investigation of the kinetics of phosphate incorporation into their precursors, adenosine 5'-triphosphate and guanosine 5'-triphosphate, allowed calculation of the average half-lives of these termini, which were found to be approximately 170 s at 21.5 C for both. The ratio between the number of nucleotides incorporated into the interior of RNA chains per second and the number of termini synthesized per second was calculated by several methods and found to be between 4,000 and 8,000. Assuming that the initiation of synthesis of a RNA chain by deoxyribonucleic acid-dependent RNA polymerase always produces a triphosphate termini and that some termini do not have half-lives so short as to not be seen in this study (less than 10 s), this is the apparent average length of the transcriptional unit. The implication of these findings to the genetic organization of transfer RNA genes is discussed.
研究了放射性磷酸盐掺入细菌核糖核酸（RNA）的腺苷和鸟苷三磷酸末端的动力学。先前对磷酸盐掺入其前体腺苷5'-三磷酸和鸟苷5'-三磷酸的动力学研究中获得的知识，使得能够计算这些末端的平均半衰期，发现在21.5℃时两者的平均半衰期约为170秒。通过几种方法计算了每秒掺入RNA链内部的核苷酸数量与每秒合成的末端数量之间的比率，发现该比率在4000至8000之间。假设依赖脱氧核糖核酸的RNA聚合酶合成RNA链的起始总是产生一个三磷酸末端，并且某些末端的半衰期不会短到在本研究中无法观察到（小于10秒），这就是转录单位的表观平均长度。讨论了这些发现对转移RNA基因遗传组织的影响。
