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含有真核生物起始因子eIF-2的核糖体后复合物。

Postribosomal complexes containing eukaryotic initiation factor eIF-2.

作者信息

Amesz H, Haubrich T, Voorma H O

出版信息

Mol Biol Rep. 1979 May 31;5(1-2):121-5. doi: 10.1007/BF00777498.

DOI:10.1007/BF00777498
PMID:460180
Abstract

Eukaryotic initiation factors are found in the post-ribosomal subunits. We have analyzed the factor activities from the supernatant by means of zonal centrifugation followed by Sepharose-heparin affinity chromatography. They exist both as free factors, sedimenting in a broad range from 4 to 7S, and complexed with other protein(s) with a sedimentation value of 16-20S. This complexed fraction contains besides eIF-2 another activity which exhibits a profound stimulation on amino acid incorporation in crude lysates and appears to counteract the heme-regulated inhibitor.

摘要

真核生物起始因子存在于核糖体后亚基中。我们通过区带离心，然后进行琼脂糖-肝素亲和层析，分析了上清液中的因子活性。它们既以游离因子形式存在，沉降范围在4至7S之间，也与其他蛋白质形成复合物，沉降值为16 - 20S。除了真核生物起始因子2（eIF-2）外，这个复合组分还含有另一种活性物质，它对粗裂解物中的氨基酸掺入有显著刺激作用，并且似乎能对抗血红素调节抑制剂。

相似文献

1
Postribosomal complexes containing eukaryotic initiation factor eIF-2.含有真核生物起始因子eIF-2的核糖体后复合物。
Mol Biol Rep. 1979 May 31;5(1-2):121-5. doi: 10.1007/BF00777498.
2
A comparison of the initiation factors of eukaryotic protein synthesis from ribosomes and from the postribosomal supernatant.
Eur J Biochem. 1979 Aug 1;98(2):329-37. doi: 10.1111/j.1432-1033.1979.tb13192.x.
3
Protein synthesis in rabbit reticulocytes: characteristics of a postribosomal supernatant factor that reverses inhibition of protein synthesis in heme-deficient lysates and inhibition of ternary complex (Met-tRNAfMet.eIF-2.GTP) formation by heme-regulated inhibitor.兔网织红细胞中的蛋白质合成：一种核糖体后上清因子的特性，该因子可逆转血红素缺乏裂解物中蛋白质合成的抑制以及血红素调节抑制剂对三元复合物（Met-tRNAfMet.eIF-2.GTP）形成的抑制。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5490-4. doi: 10.1073/pnas.76.11.5490.
4
Characterization of eukaryotic initiation factor 5 from rabbit reticulocytes. Evidence that the initiation factor is a monomeric protein of Mr of about 58,000-62,000.兔网织红细胞中真核起始因子5的特性。有证据表明该起始因子是一种分子量约为58,000 - 62,000的单体蛋白。
J Biol Chem. 1987 Oct 15;262(29):14222-7.
5
Purification and characterization of protein synthesis initiation factor eIF-4E from the yeast Saccharomyces cerevisiae.酿酒酵母蛋白质合成起始因子eIF-4E的纯化与特性分析
Biochemistry. 1985 Oct 22;24(22):6085-9. doi: 10.1021/bi00343a009.
6
Regulation of protein synthesis in rabbit reticulocyte lysates. Phosphorylation of eIF-2 does not inhibit its capacity to recycle.兔网织红细胞裂解物中蛋白质合成的调控。真核起始因子2（eIF-2）的磷酸化并不抑制其循环利用的能力。
Eur J Biochem. 1980 Mar;104(2):501-9. doi: 10.1111/j.1432-1033.1980.tb04452.x.
7
Protein synthesis in rabbit reticulocytes: characteristics of the protein factor RF that reverses inhibition of protein synthesis in heme-deficient reticulocyte lysates.兔网织红细胞中的蛋白质合成：逆转血红素缺乏的网织红细胞裂解物中蛋白质合成抑制作用的蛋白质因子RF的特性。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6517-21. doi: 10.1073/pnas.79.21.6517.
8
Protein synthesis in rabbit reticulocytes: a study of the mechanism of action of the protein factor RF that reverses protein synthesis inhibition in heme-deficient reticulocyte lysates.兔网织红细胞中的蛋白质合成：对蛋白质因子RF作用机制的研究，该因子可逆转血红素缺乏的网织红细胞裂解物中的蛋白质合成抑制。
Proc Natl Acad Sci U S A. 1984 Sep;81(17):5379-83. doi: 10.1073/pnas.81.17.5379.
9
Regulation of protein synthesis in rabbit reticulocyte lysates: additional initiation factor required for formation of ternary complex (eIF-2.GTP.Met-tRNAf) and demonstration of inhibitory effect of heme-regulated protein kinase.兔网织红细胞裂解物中蛋白质合成的调控：三元复合物（eIF-2·GTP·Met-tRNAf）形成所需的额外起始因子以及血红素调节蛋白激酶抑制作用的证明。
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1079-83. doi: 10.1073/pnas.76.3.1079.
10
Initiation factor eIF-2 from wheat germ. Purification, functional comparison to eIF-2 from rabbit reticulocytes and phosphorylation of its subunits.来自小麦胚芽的起始因子eIF-2。纯化、与兔网织红细胞的eIF-2进行功能比较及其亚基的磷酸化
Eur J Biochem. 1980 Feb;104(1):109-17. doi: 10.1111/j.1432-1033.1980.tb04406.x.

本文引用的文献

1
A protein of molecular weight 78,000 bound to the polyadenylate region of eukaryotic messenger RNAs.一种分子量为78,000的蛋白质与真核生物信使核糖核酸的聚腺苷酸区域结合。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):924-8. doi: 10.1073/pnas.70.3.924.
2
Purification and physical properties of homogeneous initiation factor MP from rabbit reticulocytes.兔网织红细胞中均一起始因子MP的纯化及物理性质
J Biol Chem. 1975 Dec 10;250(23):9067-75.
3
Purification and characterization of homogeneous initiation factor M2A from rabbit reticulocytes.兔网织红细胞中均一起始因子M2A的纯化与特性分析
J Biol Chem. 1975 Jul 25;250(14):5556-62.
4
Purification and characterization of initiation factor IF-E3 from rabbit reticulocytes.兔网织红细胞起始因子IF-E3的纯化与特性分析
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3005-9. doi: 10.1073/pnas.73.9.3005.
5
Purification and characterization of two initiation factors required for maximal activity of a highly fractionated globin mRNA translation system.高度分级分离的珠蛋白mRNA翻译系统最大活性所需的两种起始因子的纯化与特性分析
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2584-8. doi: 10.1073/pnas.73.8.2584.
6
Nonribosomal proteins associated with eukaryotic native small ribosomal subunits.与真核生物天然小核糖体亚基相关的非核糖体蛋白。
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3392-6. doi: 10.1073/pnas.72.9.3392.
7
Interaction of poly(A) and mRNA with eukaryotic initiator met-tRNA-f binding factor: identification of this activity on reticulocyte ribonucleic acid protein particles.聚腺苷酸（poly(A)）与真核起始甲硫氨酰 - tRNA - f结合因子与mRNA的相互作用：在网织红细胞核糖核酸蛋白颗粒上对该活性的鉴定。
Proc Natl Acad Sci U S A. 1975 Mar;72(3):1021-5. doi: 10.1073/pnas.72.3.1021.
8
Purification and characterization of initiation factor IF-E2 from rabbit reticulocytes.兔网织红细胞起始因子IF-E2的纯化与特性分析
J Biol Chem. 1976 Dec 10;251(23):7675-81.
9
Characterization of the association of specific proteins with messenger ribonucleic acid.
Biochemistry. 1976 Aug 10;15(16):3523-8. doi: 10.1021/bi00661a020.
10
Initiation of protein synthesis in eukaryotes. Binding to Sepharose-heparin and partial purification of initiation factors from Krebs II ascites cells.真核生物中蛋白质合成的起始。与琼脂糖-肝素结合及从克雷布斯II腹水细胞中对起始因子的部分纯化。
Eur J Biochem. 1977 May 16;75(2):455-64. doi: 10.1111/j.1432-1033.1977.tb11548.x.